Improvement in the relationship between flow to ischemic myocardium and the extent of necrosis with glycolytic intermediates that decrease blood oxygen affinity in dogs

George Pantely, A. A. Oyama, J. Metcalfe, M. S. Lawson, J. E. Welch

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    19 Citations (Scopus)

    Abstract

    Reducing blood oxygen affinity may enhance myocardial oxygen delivery during ischemia. We evaluated this hypothesis in awake, preveiously instrumented dogs that received a 20 ml/kg infusion of a solution of dihydroxyacetone, phosphate, and pyruvate after acute occlusion of either the left anterior descending of circumflex coronary artery. This infusion reduced blood oxygen affinity (↓BOA) after 2 hours; the P 50 increased from 29.9 ± 0.7 torr (mean ± SD) to 32.1 ± 0.6 torr; P <0.01 (BOA group). Four dogs received 20 ml/kg of phosphate and pyruvate solution to assess volume effects (V group), and five dogs were controls (C group). The 2-hour P 50 values in V and C were unchanged. Regional flow (15-μm spheres) reduction 2 hours postocclusion was compared to the percent tissue infarcted determined by histology 7-9 days after occlusion for multiple samples from the endocardial layer of the left ventricle. When flow was less than 40% of normal, V and C had 55% infarction while ↓BOA had 37% (P <0.05); at flow less than 20% of normal, V and C had 79% infarction while ↓BOA had 38% (P <0.001); and at less than 10% of normal, V and C had 87% and 94% infarction, respectively, while ↓BOA had 56% (P <0.001). Reducing blood oxygen affinity after coronary artery occlusion significantly decreased the extent of myocardial necrosis for the same degree of ischemia. Reducing BOA may increase oxygen delivery to ischemic myocardium when flow is restricted.

    Original languageEnglish (US)
    Pages (from-to)395-404
    Number of pages10
    JournalCirculation Research
    Volume49
    Issue number2
    StatePublished - 1981

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    Myocardium
    Necrosis
    Dogs
    Oxygen
    Infarction
    Pyruvic Acid
    Coronary Vessels
    Ischemia
    Dihydroxyacetone Phosphate
    Coronary Occlusion
    Heart Ventricles
    Histology
    Phosphates
    Control Groups

    ASJC Scopus subject areas

    • Physiology
    • Cardiology and Cardiovascular Medicine

    Cite this

    Improvement in the relationship between flow to ischemic myocardium and the extent of necrosis with glycolytic intermediates that decrease blood oxygen affinity in dogs. / Pantely, George; Oyama, A. A.; Metcalfe, J.; Lawson, M. S.; Welch, J. E.

    In: Circulation Research, Vol. 49, No. 2, 1981, p. 395-404.

    Research output: Contribution to journalArticle

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