Immunophenotypic and intracellular cytokine profile of Indian patients with tuberculosis with and without human immunodeficiency virus co-infection.

Ajay Wanchu, A. Bhatnagar, J. Talreja, S. Sapra, B. S. Suryanarayana, P. Suresh

Research output: Contribution to journalArticle

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Abstract

BACKGROUND: Tuberculosis (TB) occurs in more than 50% of human immunodeficiency virus (HIV) infected Indian patients. This study was carried out to determine the immunophenotypic and intracellular cytokine profile of patients with HIV-TB co-infection. PATIENTS AND METHODS: Fifteen patients with HIV-TB co-infection and 15 each with TB alone and healthy individuals were studied. Immunophenotypic analysis and intracellular cytokines were measured using appropriate antibodies on a flowcytometer. RESULTS: Percentage of CD3+ did not differ significantly in the three groups. The ratio of CD4+ : CD8+ was reversed among patients with TB and HIV-TB. CD19+ and CD25+ were present on fewer cells of healthy individuals but this was not statistically significant. Significantly higher percentage of cells of patients with TB and HIV-TB were CD69 positive. Interferon-gamma (INF-gamma) and tumour necrosis factor-alpha (TNF-alpha) levels are significantly reduced in the CD4+ cells of patients with HIV-TB when compared with those with TB and healthy individuals. In CD8+ cells of patients with HIV-TB, levels of TNF-alpha are higher when compared with the other two groups. Interleukin-2 (IL-2) producing cells were not significantly different in any of the above subsets. Monocytes in individuals with HIV-TB had significantly higher interleukin-6 (IL-6) and TNF-alpha. CONCLUSIONS: T-helper cells among patients with HIV-TB have significantly lower cytokine production. T-suppressor cells and monocytes produce more TNF-alpha. These findings may be significant in view of recent attempts to treat HIV-TB coinfected patients with anti-TNF therapy.

Original languageEnglish (US)
Pages (from-to)207-211
Number of pages5
JournalThe Indian journal of chest diseases & allied sciences
Volume51
Issue number4
StatePublished - Oct 2009
Externally publishedYes

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Virus Diseases
Coinfection
Tuberculosis
HIV
Cytokines
Tumor Necrosis Factor-alpha
Monocytes
CD4-CD8 Ratio
Helper-Inducer T-Lymphocytes
Interferon-gamma
Interleukin-2
Interleukin-6

ASJC Scopus subject areas

  • Medicine(all)

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Immunophenotypic and intracellular cytokine profile of Indian patients with tuberculosis with and without human immunodeficiency virus co-infection. / Wanchu, Ajay; Bhatnagar, A.; Talreja, J.; Sapra, S.; Suryanarayana, B. S.; Suresh, P.

In: The Indian journal of chest diseases & allied sciences, Vol. 51, No. 4, 10.2009, p. 207-211.

Research output: Contribution to journalArticle

Wanchu, Ajay ; Bhatnagar, A. ; Talreja, J. ; Sapra, S. ; Suryanarayana, B. S. ; Suresh, P. / Immunophenotypic and intracellular cytokine profile of Indian patients with tuberculosis with and without human immunodeficiency virus co-infection. In: The Indian journal of chest diseases & allied sciences. 2009 ; Vol. 51, No. 4. pp. 207-211.
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