Immunologic memory to PC-KLH

Participation of the Q52 V(H) gene family

Mary Stenzel-Poore, T. J. Hall, C. H. Heusser, C. H. Faust, Marvin Rittenberg

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

BALB/c mice immunized with phosphocholine-conjugated keyhole limpet hemocyanin respond with two major groups of antibodies that differ with respect to fine specificity and idiotype. Group I antibodies predominantly bear the T15 idiotype, and show appreciable affinity for the haptens PC and nitrophenyl PC (NPPC), whereas group II antibodies have appreciable affinity for NPPC only and are T15 idiotype negative. Previous studies indicated that group II binding characteristics may derive from the use of novel V gene segments not observed in group I antibodies. To determine the nature of V(H) gene usage in the group II antibody response, we examined the V(H) region of a prototype group II hybridoma, PCG1-1. The nucleotide sequence obtained from the VDJ region indicates that PCG1-1 utilizes a V(H) gene not observed in the group I response, one that belongs to the Q52 V(H) family. The PCG1-1 V(H) nucleotide sequence shares 97% identity with the myeloma M141 V(H) gene. In addition, PCG1-1 utilizes a D segment most closely related to DSP2.6 rearranged to J(H)-3. These data indicate that M141, a V(H) gene not seen in group I anti-PC antibodies is utilized by PCG1-1 to generate a PC-protein-binding group II antibody. PCG1-1 was previously shown to express the V(κ)1-3 light chain, a characteristic shared by several group II hybridomas. Furthermore, here we examined the V(H) gene rearrangements in four λ1-bearing group II hybridomas that share a common J(H) rearrangement with PCG1-1 by Southern blot analysis. A V(H)-specific probe that detects M141 V(H) rearrangements revealed that all four λ1 hybridomas as well as PCG1-1 share an identical V(H) gene rearrangement to J(H)-3. Thus the M141 V(H) gene product is able to utilize two distinct light chains to generate group II-like combining sites.

Original languageEnglish (US)
Pages (from-to)1698-1703
Number of pages6
JournalJournal of Immunology
Volume139
Issue number5
StatePublished - 1987

Fingerprint

Immunologic Memory
Hybridomas
Antibodies
Genes
Gene Rearrangement
Light
Phosphorylcholine
Haptens
Southern Blotting
Protein Binding
Antibody Formation
Anti-Idiotypic Antibodies
Binding Sites

ASJC Scopus subject areas

  • Immunology

Cite this

Stenzel-Poore, M., Hall, T. J., Heusser, C. H., Faust, C. H., & Rittenberg, M. (1987). Immunologic memory to PC-KLH: Participation of the Q52 V(H) gene family. Journal of Immunology, 139(5), 1698-1703.

Immunologic memory to PC-KLH : Participation of the Q52 V(H) gene family. / Stenzel-Poore, Mary; Hall, T. J.; Heusser, C. H.; Faust, C. H.; Rittenberg, Marvin.

In: Journal of Immunology, Vol. 139, No. 5, 1987, p. 1698-1703.

Research output: Contribution to journalArticle

Stenzel-Poore, M, Hall, TJ, Heusser, CH, Faust, CH & Rittenberg, M 1987, 'Immunologic memory to PC-KLH: Participation of the Q52 V(H) gene family', Journal of Immunology, vol. 139, no. 5, pp. 1698-1703.
Stenzel-Poore M, Hall TJ, Heusser CH, Faust CH, Rittenberg M. Immunologic memory to PC-KLH: Participation of the Q52 V(H) gene family. Journal of Immunology. 1987;139(5):1698-1703.
Stenzel-Poore, Mary ; Hall, T. J. ; Heusser, C. H. ; Faust, C. H. ; Rittenberg, Marvin. / Immunologic memory to PC-KLH : Participation of the Q52 V(H) gene family. In: Journal of Immunology. 1987 ; Vol. 139, No. 5. pp. 1698-1703.
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abstract = "BALB/c mice immunized with phosphocholine-conjugated keyhole limpet hemocyanin respond with two major groups of antibodies that differ with respect to fine specificity and idiotype. Group I antibodies predominantly bear the T15 idiotype, and show appreciable affinity for the haptens PC and nitrophenyl PC (NPPC), whereas group II antibodies have appreciable affinity for NPPC only and are T15 idiotype negative. Previous studies indicated that group II binding characteristics may derive from the use of novel V gene segments not observed in group I antibodies. To determine the nature of V(H) gene usage in the group II antibody response, we examined the V(H) region of a prototype group II hybridoma, PCG1-1. The nucleotide sequence obtained from the VDJ region indicates that PCG1-1 utilizes a V(H) gene not observed in the group I response, one that belongs to the Q52 V(H) family. The PCG1-1 V(H) nucleotide sequence shares 97{\%} identity with the myeloma M141 V(H) gene. In addition, PCG1-1 utilizes a D segment most closely related to DSP2.6 rearranged to J(H)-3. These data indicate that M141, a V(H) gene not seen in group I anti-PC antibodies is utilized by PCG1-1 to generate a PC-protein-binding group II antibody. PCG1-1 was previously shown to express the V(κ)1-3 light chain, a characteristic shared by several group II hybridomas. Furthermore, here we examined the V(H) gene rearrangements in four λ1-bearing group II hybridomas that share a common J(H) rearrangement with PCG1-1 by Southern blot analysis. A V(H)-specific probe that detects M141 V(H) rearrangements revealed that all four λ1 hybridomas as well as PCG1-1 share an identical V(H) gene rearrangement to J(H)-3. Thus the M141 V(H) gene product is able to utilize two distinct light chains to generate group II-like combining sites.",
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N2 - BALB/c mice immunized with phosphocholine-conjugated keyhole limpet hemocyanin respond with two major groups of antibodies that differ with respect to fine specificity and idiotype. Group I antibodies predominantly bear the T15 idiotype, and show appreciable affinity for the haptens PC and nitrophenyl PC (NPPC), whereas group II antibodies have appreciable affinity for NPPC only and are T15 idiotype negative. Previous studies indicated that group II binding characteristics may derive from the use of novel V gene segments not observed in group I antibodies. To determine the nature of V(H) gene usage in the group II antibody response, we examined the V(H) region of a prototype group II hybridoma, PCG1-1. The nucleotide sequence obtained from the VDJ region indicates that PCG1-1 utilizes a V(H) gene not observed in the group I response, one that belongs to the Q52 V(H) family. The PCG1-1 V(H) nucleotide sequence shares 97% identity with the myeloma M141 V(H) gene. In addition, PCG1-1 utilizes a D segment most closely related to DSP2.6 rearranged to J(H)-3. These data indicate that M141, a V(H) gene not seen in group I anti-PC antibodies is utilized by PCG1-1 to generate a PC-protein-binding group II antibody. PCG1-1 was previously shown to express the V(κ)1-3 light chain, a characteristic shared by several group II hybridomas. Furthermore, here we examined the V(H) gene rearrangements in four λ1-bearing group II hybridomas that share a common J(H) rearrangement with PCG1-1 by Southern blot analysis. A V(H)-specific probe that detects M141 V(H) rearrangements revealed that all four λ1 hybridomas as well as PCG1-1 share an identical V(H) gene rearrangement to J(H)-3. Thus the M141 V(H) gene product is able to utilize two distinct light chains to generate group II-like combining sites.

AB - BALB/c mice immunized with phosphocholine-conjugated keyhole limpet hemocyanin respond with two major groups of antibodies that differ with respect to fine specificity and idiotype. Group I antibodies predominantly bear the T15 idiotype, and show appreciable affinity for the haptens PC and nitrophenyl PC (NPPC), whereas group II antibodies have appreciable affinity for NPPC only and are T15 idiotype negative. Previous studies indicated that group II binding characteristics may derive from the use of novel V gene segments not observed in group I antibodies. To determine the nature of V(H) gene usage in the group II antibody response, we examined the V(H) region of a prototype group II hybridoma, PCG1-1. The nucleotide sequence obtained from the VDJ region indicates that PCG1-1 utilizes a V(H) gene not observed in the group I response, one that belongs to the Q52 V(H) family. The PCG1-1 V(H) nucleotide sequence shares 97% identity with the myeloma M141 V(H) gene. In addition, PCG1-1 utilizes a D segment most closely related to DSP2.6 rearranged to J(H)-3. These data indicate that M141, a V(H) gene not seen in group I anti-PC antibodies is utilized by PCG1-1 to generate a PC-protein-binding group II antibody. PCG1-1 was previously shown to express the V(κ)1-3 light chain, a characteristic shared by several group II hybridomas. Furthermore, here we examined the V(H) gene rearrangements in four λ1-bearing group II hybridomas that share a common J(H) rearrangement with PCG1-1 by Southern blot analysis. A V(H)-specific probe that detects M141 V(H) rearrangements revealed that all four λ1 hybridomas as well as PCG1-1 share an identical V(H) gene rearrangement to J(H)-3. Thus the M141 V(H) gene product is able to utilize two distinct light chains to generate group II-like combining sites.

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