Immunofluorescent detection of histone 2B on metaphase chromosomes using flow cytometry

Barb Trask, Ger van den Engh, Joe Gray, Marty Vanderlaan, Bryan Turner

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A monoclonal antibody against histone 2B (anti-H2B) was used as a reagent to stain isolated chromosomes for analysis using flow cytometry. Chromosome suspensions were treated with a mouse monoclonal antibody specific for the histone 2B (clone HBC-7) and then with a fluorescein-labeled goat anti-mouse-IgM antibody. The chromosomes were also stained for DNA content with either Hoechst 33258 or propidium iodide. The amount of antibody and the amount of DNA-specific stain bound to each chromosome were measured simultaneously using flow cytometry. The order of the steps in the staining protocol is important. Propidium iodide prevents anti-H2B from binding to chromosomes, and therefore must be added only after antibody labeling is completed. In contrast, the addition of Hoechst 33258 before antibody labeling reduces antibody binding by only 20%-30%. Binding of anti-H2B was proportional to the DNA content of both human and Chinese hamster chromosomes. Human chromosomes bind an average of three to four times more anti-H2B than do Chinese hamster or mouse chromosomes of the same DNA content. This was determined by analyzing mixtures of human and Chinese hamster chromosomes and human and mouse chromosomes. The results demonstrate that it is possible to label the proteins of chromosomes in suspension with fluorescent antibodies and to use these reagents for the analysis of chromosome structure by flow cytometry.

Original languageEnglish (US)
Pages (from-to)295-302
Number of pages8
JournalChromosoma
Volume90
Issue number4
DOIs
StatePublished - Oct 1984
Externally publishedYes

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Metaphase
Histones
Flow Cytometry
Chromosomes
Antibodies
Cricetulus
Bisbenzimidazole
Propidium
DNA
Human Chromosomes
Suspensions
Coloring Agents
Monoclonal Antibodies
Chromosome Structures
Fluorescein
Goats
Clone Cells
Staining and Labeling

ASJC Scopus subject areas

  • Genetics

Cite this

Immunofluorescent detection of histone 2B on metaphase chromosomes using flow cytometry. / Trask, Barb; van den Engh, Ger; Gray, Joe; Vanderlaan, Marty; Turner, Bryan.

In: Chromosoma, Vol. 90, No. 4, 10.1984, p. 295-302.

Research output: Contribution to journalArticle

Trask, B, van den Engh, G, Gray, J, Vanderlaan, M & Turner, B 1984, 'Immunofluorescent detection of histone 2B on metaphase chromosomes using flow cytometry', Chromosoma, vol. 90, no. 4, pp. 295-302. https://doi.org/10.1007/BF00287038
Trask, Barb ; van den Engh, Ger ; Gray, Joe ; Vanderlaan, Marty ; Turner, Bryan. / Immunofluorescent detection of histone 2B on metaphase chromosomes using flow cytometry. In: Chromosoma. 1984 ; Vol. 90, No. 4. pp. 295-302.
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