Immunocytochemical methods for demonstrating canine distemper virus antigen in aldehyde-fixed paraffin-embedded tissue

Michael K. Axthelm, Steven Krakowka

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

The effects of enzymatic digestion, sodium borohydride reduction, acids used in décalcification procedures and techniques for inactivation of endogenous peroxidase were sequentially evaluated for their effect on the immunoreactivity of canine distemper virus in aldehyde-fixed paraffin-embedded tissue. Enzyme digestion improved immunoreactivity while sodium borohydride reduced background staining. Paraformaldehyde-glutaraldehyde-fixed tissues required thioglycolic acid treatment prior to enzyme digestion and sodium borohydride reduction to obtain results comparable to results obtained in formalin-fixed tissues. Detailed protocols for indirect immunofluorescence and the avidin-biotin-peroxidase complex procedure are provided.

Original languageEnglish (US)
Pages (from-to)215-229
Number of pages15
JournalJournal of Virological Methods
Volume13
Issue number3
DOIs
StatePublished - Jun 1986
Externally publishedYes

Keywords

  • aldehyde fixation
  • canine distemper virus
  • immunocytochemistry
  • immunofluorescence technique
  • immunoperoxidase
  • technique

ASJC Scopus subject areas

  • Virology

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