Abstract
The effects of enzymatic digestion, sodium borohydride reduction, acids used in décalcification procedures and techniques for inactivation of endogenous peroxidase were sequentially evaluated for their effect on the immunoreactivity of canine distemper virus in aldehyde-fixed paraffin-embedded tissue. Enzyme digestion improved immunoreactivity while sodium borohydride reduced background staining. Paraformaldehyde-glutaraldehyde-fixed tissues required thioglycolic acid treatment prior to enzyme digestion and sodium borohydride reduction to obtain results comparable to results obtained in formalin-fixed tissues. Detailed protocols for indirect immunofluorescence and the avidin-biotin-peroxidase complex procedure are provided.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 215-229 |
| Number of pages | 15 |
| Journal | Journal of Virological Methods |
| Volume | 13 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1986 |
| Externally published | Yes |
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Keywords
- aldehyde fixation
- canine distemper virus
- immunocytochemistry
- immunofluorescence technique
- immunoperoxidase
- technique
ASJC Scopus subject areas
- Virology
Cite this
Immunocytochemical methods for demonstrating canine distemper virus antigen in aldehyde-fixed paraffin-embedded tissue. / Axthelm, Michael; Krakowka, Steven.
In: Journal of Virological Methods, Vol. 13, No. 3, 1986, p. 215-229.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunocytochemical methods for demonstrating canine distemper virus antigen in aldehyde-fixed paraffin-embedded tissue
AU - Axthelm, Michael
AU - Krakowka, Steven
PY - 1986
Y1 - 1986
N2 - The effects of enzymatic digestion, sodium borohydride reduction, acids used in décalcification procedures and techniques for inactivation of endogenous peroxidase were sequentially evaluated for their effect on the immunoreactivity of canine distemper virus in aldehyde-fixed paraffin-embedded tissue. Enzyme digestion improved immunoreactivity while sodium borohydride reduced background staining. Paraformaldehyde-glutaraldehyde-fixed tissues required thioglycolic acid treatment prior to enzyme digestion and sodium borohydride reduction to obtain results comparable to results obtained in formalin-fixed tissues. Detailed protocols for indirect immunofluorescence and the avidin-biotin-peroxidase complex procedure are provided.
AB - The effects of enzymatic digestion, sodium borohydride reduction, acids used in décalcification procedures and techniques for inactivation of endogenous peroxidase were sequentially evaluated for their effect on the immunoreactivity of canine distemper virus in aldehyde-fixed paraffin-embedded tissue. Enzyme digestion improved immunoreactivity while sodium borohydride reduced background staining. Paraformaldehyde-glutaraldehyde-fixed tissues required thioglycolic acid treatment prior to enzyme digestion and sodium borohydride reduction to obtain results comparable to results obtained in formalin-fixed tissues. Detailed protocols for indirect immunofluorescence and the avidin-biotin-peroxidase complex procedure are provided.
KW - aldehyde fixation
KW - canine distemper virus
KW - immunocytochemistry
KW - immunofluorescence technique
KW - immunoperoxidase
KW - technique
UR - http://www.scopus.com/inward/record.url?scp=0022574301&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022574301&partnerID=8YFLogxK
U2 - 10.1016/0166-0934(86)90016-9
DO - 10.1016/0166-0934(86)90016-9
M3 - Article
C2 - 3090088
AN - SCOPUS:0022574301
VL - 13
SP - 215
EP - 229
JO - Journal of Virological Methods
JF - Journal of Virological Methods
SN - 0166-0934
IS - 3
ER -