Immune competency of a Hairless mouse strain for improved preclinical studies in genetically engineered mice

Beverly S. Schaffer, Marcia H. Grayson, Joy M. Wortham, Courtney B. Kubicek, Amanda T. McCleish, Suresh I. Prajapati, Laura D. Nelon, Michelle M. Brady, Inkyung Jung, Tohru Hosoyama, Leslea M. Sarro, Martha A. Hanes, Brian P. Rubin, Joel E. Michalek, Charles B. Clifford, Anthony J. Infante, Charles Keller

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Genetically engineered mouse models (GEMM) of cancer are of increasing value to preclinical therapeutics. Optical imaging is a cost-effective method of assessing deep-seated tumor growth in GEMMs whose tumors can be encoded to express luminescent or fluorescent reporters, although reporter signal attenuation would be improved if animals were fur-free. In this study, we sought to determine whether hereditable furlessness resulting from a hypomorphic mutation in the Hairless gene would or would not also affect immune competence. By assessing humoral and cellular immunity of the SKH1 mouse line bearing the hypomorphic Hairless mutation, we determined that blood counts, immunoglobulin levels, and CD4+ and CD8+ T cells were comparable between SKH1 and the C57Bl/6 strain. On examination of T-cell subsets, statistically significant differences in naïve T cells (1.7 versus 3.4 × 10 5 cells/spleen in SKH1 versus C57Bl/6, P = 0.008) and memory T cells (1.4 versus 0.13 × 10 6 cells/spleen in SKH1 versus C57Bl/6, P = 0.008) were detected. However, the numerical differences did not result in altered T-cell functional response to antigen rechallenge (keyhole limpet hemocyanin) in a lymph node cell in vitro proliferative assay. Furthermore, interbreeding the SKH1 mouse line to a rhabdomyosarcoma GEMM showed preserved antitumor responses of CD56+ natural killer cells and CD163+ macrophages, without any differences in tumor pathology. The fur-free GEMM was also especially amenable to multiplex optical imaging. Thus, SKH1 represents an immune competent, fur-free mouse strain that may be of use for interbreeding to other genetically engineered mouse models of cancer for improved preclinical studies.

Original languageEnglish (US)
Pages (from-to)2354-2364
Number of pages11
JournalMolecular Cancer Therapeutics
Volume9
Issue number8
DOIs
StatePublished - Aug 2010
Externally publishedYes

Fingerprint

Hairless Mouse
T-Lymphocytes
Optical Imaging
Neoplasms
Spleen
Mutation
Rhabdomyosarcoma
T-Lymphocyte Subsets
Humoral Immunity
Cellular Immunity
Natural Killer Cells
Hair
Mental Competency
Immunoglobulins
Lymph Nodes
Macrophages
Pathology
Antigens
Costs and Cost Analysis
Growth

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Schaffer, B. S., Grayson, M. H., Wortham, J. M., Kubicek, C. B., McCleish, A. T., Prajapati, S. I., ... Keller, C. (2010). Immune competency of a Hairless mouse strain for improved preclinical studies in genetically engineered mice. Molecular Cancer Therapeutics, 9(8), 2354-2364. https://doi.org/10.1158/1535-7163.MCT-10-0207

Immune competency of a Hairless mouse strain for improved preclinical studies in genetically engineered mice. / Schaffer, Beverly S.; Grayson, Marcia H.; Wortham, Joy M.; Kubicek, Courtney B.; McCleish, Amanda T.; Prajapati, Suresh I.; Nelon, Laura D.; Brady, Michelle M.; Jung, Inkyung; Hosoyama, Tohru; Sarro, Leslea M.; Hanes, Martha A.; Rubin, Brian P.; Michalek, Joel E.; Clifford, Charles B.; Infante, Anthony J.; Keller, Charles.

In: Molecular Cancer Therapeutics, Vol. 9, No. 8, 08.2010, p. 2354-2364.

Research output: Contribution to journalArticle

Schaffer, BS, Grayson, MH, Wortham, JM, Kubicek, CB, McCleish, AT, Prajapati, SI, Nelon, LD, Brady, MM, Jung, I, Hosoyama, T, Sarro, LM, Hanes, MA, Rubin, BP, Michalek, JE, Clifford, CB, Infante, AJ & Keller, C 2010, 'Immune competency of a Hairless mouse strain for improved preclinical studies in genetically engineered mice', Molecular Cancer Therapeutics, vol. 9, no. 8, pp. 2354-2364. https://doi.org/10.1158/1535-7163.MCT-10-0207
Schaffer, Beverly S. ; Grayson, Marcia H. ; Wortham, Joy M. ; Kubicek, Courtney B. ; McCleish, Amanda T. ; Prajapati, Suresh I. ; Nelon, Laura D. ; Brady, Michelle M. ; Jung, Inkyung ; Hosoyama, Tohru ; Sarro, Leslea M. ; Hanes, Martha A. ; Rubin, Brian P. ; Michalek, Joel E. ; Clifford, Charles B. ; Infante, Anthony J. ; Keller, Charles. / Immune competency of a Hairless mouse strain for improved preclinical studies in genetically engineered mice. In: Molecular Cancer Therapeutics. 2010 ; Vol. 9, No. 8. pp. 2354-2364.
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