Immobilized platelets support human colon carcinoma cell tethering, rolling and firm adhesion under dynamic flow conditions

Owen McCarty, Konstantinos Konstantopoulos

Research output: Contribution to journalArticle

Abstract

A study was designed to analyze the ability of a human colon carcinoma cell line, LS174T, to bind to platelets under flow, and to identify the receptors involved. Immobilized platelets support LS174T cell adhesion at wall shear stresses up to 1.4 dyn/cm2. Platelet P-selectin mediates LS174T cell tethering and rolling in a PSGL-1 and CD24-independent manner. Platelet GPIIbIIIa integrins appear to be capable of directly capturing LS174T cells from the fluid stream, and also convert instantaneously transient tethers initiated by P-selectin into stable adhesion.

Original languageEnglish (US)
JournalAnnals of Biomedical Engineering
Volume28
Issue numberSUPPL. 1
StatePublished - 2000
Externally publishedYes

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Platelets
Adhesion
Cells
Cell adhesion
Shear stress
Fluids
Selectins

ASJC Scopus subject areas

  • Biomedical Engineering

Cite this

Immobilized platelets support human colon carcinoma cell tethering, rolling and firm adhesion under dynamic flow conditions. / McCarty, Owen; Konstantopoulos, Konstantinos.

In: Annals of Biomedical Engineering, Vol. 28, No. SUPPL. 1, 2000.

Research output: Contribution to journalArticle

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