Abstract
Rapid one-step modification of thrombomodulin with alkylamine derivatives such as azide, biotin, and PEG is achieved using an evolved sortase (eSrtA) mutant. The feasibility of a point-of-care scheme is demonstrated herein to site-specifically immobilize azido-thrombomodulin on sterilized commercial ePTFE vascular grafts, which exhibit superior thromboresistance compared with commercial heparin-coated grafts in a primate model of acute graft thrombosis.
Original language | English (US) |
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Pages (from-to) | 30-35 |
Number of pages | 6 |
Journal | Advanced Healthcare Materials |
Volume | 3 |
Issue number | 1 |
DOIs | |
State | Published - Jan 2014 |
Keywords
- Antifouling
- Biocompatible materials
- Biomedical applications
- Thrombomodulin
- Thrombosis
ASJC Scopus subject areas
- Biomaterials
- Biomedical Engineering
- Pharmaceutical Science