Immobilization of Actively Thromboresistant Assemblies on Sterile Blood-Contacting Surfaces

Zheng Qu, Venkat Krishnamurthy, Carolyn A. Haller, Brent M. Dorr, Ulla M. Marzec, Sawan Hurst, Monica Hinds, Stephen R. Hanson, David R. Liu, Elliot L. Chaikof

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Rapid one-step modification of thrombomodulin with alkylamine derivatives such as azide, biotin, and PEG is achieved using an evolved sortase (eSrtA) mutant. The feasibility of a point-of-care scheme is demonstrated herein to site-specifically immobilize azido-thrombomodulin on sterilized commercial ePTFE vascular grafts, which exhibit superior thromboresistance compared with commercial heparin-coated grafts in a primate model of acute graft thrombosis.

Original languageEnglish (US)
Pages (from-to)30-35
Number of pages6
JournalAdvanced healthcare materials
Volume3
Issue number1
DOIs
StatePublished - Jan 2014

Fingerprint

Grafts
Immobilization
Thrombomodulin
Blood
Transplants
Point-of-Care Systems
Azides
Biotin
Primates
Polyethylene glycols
Blood Vessels
Heparin
Thrombosis
Derivatives

Keywords

  • Antifouling
  • Biocompatible materials
  • Biomedical applications
  • Thrombomodulin
  • Thrombosis

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Pharmaceutical Science

Cite this

Qu, Z., Krishnamurthy, V., Haller, C. A., Dorr, B. M., Marzec, U. M., Hurst, S., ... Chaikof, E. L. (2014). Immobilization of Actively Thromboresistant Assemblies on Sterile Blood-Contacting Surfaces. Advanced healthcare materials, 3(1), 30-35. https://doi.org/10.1002/adhm.201300110

Immobilization of Actively Thromboresistant Assemblies on Sterile Blood-Contacting Surfaces. / Qu, Zheng; Krishnamurthy, Venkat; Haller, Carolyn A.; Dorr, Brent M.; Marzec, Ulla M.; Hurst, Sawan; Hinds, Monica; Hanson, Stephen R.; Liu, David R.; Chaikof, Elliot L.

In: Advanced healthcare materials, Vol. 3, No. 1, 01.2014, p. 30-35.

Research output: Contribution to journalArticle

Qu, Z, Krishnamurthy, V, Haller, CA, Dorr, BM, Marzec, UM, Hurst, S, Hinds, M, Hanson, SR, Liu, DR & Chaikof, EL 2014, 'Immobilization of Actively Thromboresistant Assemblies on Sterile Blood-Contacting Surfaces', Advanced healthcare materials, vol. 3, no. 1, pp. 30-35. https://doi.org/10.1002/adhm.201300110
Qu, Zheng ; Krishnamurthy, Venkat ; Haller, Carolyn A. ; Dorr, Brent M. ; Marzec, Ulla M. ; Hurst, Sawan ; Hinds, Monica ; Hanson, Stephen R. ; Liu, David R. ; Chaikof, Elliot L. / Immobilization of Actively Thromboresistant Assemblies on Sterile Blood-Contacting Surfaces. In: Advanced healthcare materials. 2014 ; Vol. 3, No. 1. pp. 30-35.
@article{142414849e5a4e1b9f9ea29e42b312d3,
title = "Immobilization of Actively Thromboresistant Assemblies on Sterile Blood-Contacting Surfaces",
abstract = "Rapid one-step modification of thrombomodulin with alkylamine derivatives such as azide, biotin, and PEG is achieved using an evolved sortase (eSrtA) mutant. The feasibility of a point-of-care scheme is demonstrated herein to site-specifically immobilize azido-thrombomodulin on sterilized commercial ePTFE vascular grafts, which exhibit superior thromboresistance compared with commercial heparin-coated grafts in a primate model of acute graft thrombosis.",
keywords = "Antifouling, Biocompatible materials, Biomedical applications, Thrombomodulin, Thrombosis",
author = "Zheng Qu and Venkat Krishnamurthy and Haller, {Carolyn A.} and Dorr, {Brent M.} and Marzec, {Ulla M.} and Sawan Hurst and Monica Hinds and Hanson, {Stephen R.} and Liu, {David R.} and Chaikof, {Elliot L.}",
year = "2014",
month = "1",
doi = "10.1002/adhm.201300110",
language = "English (US)",
volume = "3",
pages = "30--35",
journal = "Advanced healthcare materials",
issn = "2192-2640",
publisher = "John Wiley and Sons Ltd",
number = "1",

}

TY - JOUR

T1 - Immobilization of Actively Thromboresistant Assemblies on Sterile Blood-Contacting Surfaces

AU - Qu, Zheng

AU - Krishnamurthy, Venkat

AU - Haller, Carolyn A.

AU - Dorr, Brent M.

AU - Marzec, Ulla M.

AU - Hurst, Sawan

AU - Hinds, Monica

AU - Hanson, Stephen R.

AU - Liu, David R.

AU - Chaikof, Elliot L.

PY - 2014/1

Y1 - 2014/1

N2 - Rapid one-step modification of thrombomodulin with alkylamine derivatives such as azide, biotin, and PEG is achieved using an evolved sortase (eSrtA) mutant. The feasibility of a point-of-care scheme is demonstrated herein to site-specifically immobilize azido-thrombomodulin on sterilized commercial ePTFE vascular grafts, which exhibit superior thromboresistance compared with commercial heparin-coated grafts in a primate model of acute graft thrombosis.

AB - Rapid one-step modification of thrombomodulin with alkylamine derivatives such as azide, biotin, and PEG is achieved using an evolved sortase (eSrtA) mutant. The feasibility of a point-of-care scheme is demonstrated herein to site-specifically immobilize azido-thrombomodulin on sterilized commercial ePTFE vascular grafts, which exhibit superior thromboresistance compared with commercial heparin-coated grafts in a primate model of acute graft thrombosis.

KW - Antifouling

KW - Biocompatible materials

KW - Biomedical applications

KW - Thrombomodulin

KW - Thrombosis

UR - http://www.scopus.com/inward/record.url?scp=84891788106&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84891788106&partnerID=8YFLogxK

U2 - 10.1002/adhm.201300110

DO - 10.1002/adhm.201300110

M3 - Article

VL - 3

SP - 30

EP - 35

JO - Advanced healthcare materials

JF - Advanced healthcare materials

SN - 2192-2640

IS - 1

ER -