IL-1β potentiates the acetylcholine induced release of vasopressin from the hypothalamus in vitro, but not from the amygdala

Jacob Raber, E. M. Pich, G. F. Koob, F. E. Bloom

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Abstract

In addition to the magnocellular hypothalamic nuclei, arginine vasopressin (AVP)-containing neurons have also been identified in limbic structures, including the hippocampus and amygdala. In the present study, we compared the qualitative properties of the in vitro release of AVP from the dissected hypothalamus with the in vitro release from the dissected amygdala and used these release systems to evaluate the interactions with neurotransmitters and cytokines. The areas of the paraventricular nucleus and supraoptic nucleus that contain the AVP neurons and that receive cholinergic innervation are also interleukin (IL)-1β immunoreactive. Acetylcholine or high KCl (60 mM) induces AVP release in both regions, and the AVP release is calcium dependent. Acetylcholine-induced AVP release is antagonized by atropine or mecamylamine, indicating that both muscarinic and nicotinic receptors are mediating the cholinergic effect in these brain regions. IL-1β (100 U/ml) had no effect on the basal AVP release from the hypothalamus, but significantly potentiated the acetylcholine-induced AVP release, lowering the threshold from 500 to 100 nM. This effect was completely blocked in the presence of neutralizing antibodies to IL-1β, atropine (10 μM) or mecamylamine (10 μM). IL-6, like IL-1β, also potentiated acetylcholine-induced AVP release, but to a lesser extent. Neither tumor necrosis factor-α nor interferon-γ had any effect on the basal or acetylcholine-induced AVP release from the hypothalamus. None of the cytokines tested had any effect on the basal or acetylcholine-induced AVP release from the amygdala. Our results suggest a hypothalamic site of action of IL-1β and IL-6 on the acetylcholine-induced AVP release. The stimulatory effects of IL-1 and IL-6 on adrenocorticotropin release have been ascribed to an increased release of corticotropin-releasing factor (CRF). These data further suggest that, in addition to CRF, AVP plays a role in the bidirectional communication between neuroendocrine and immune systems. Understanding the mode of interaction between IL-1β and IL-6 with AVP could clarify pathophysiologic or toxic effects of high brain levels of these cytokines.

Original languageEnglish (US)
Pages (from-to)208-217
Number of pages10
JournalNeuroendocrinology
Volume59
Issue number3
Publication statusPublished - 1994
Externally publishedYes

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Keywords

  • Acetylcholine
  • Amygdala
  • Hypothalamus
  • Interleukin-1
  • Interleukin-6
  • Rat
  • Vasopressin

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)

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