Identification of sodium channel subtypes induced in cultured retinal pigment epithelium cells

Heather Dawes; Gail Mandel; Gary Matthews

doi:10.1017/S0952523800009536

Identification of sodium channel subtypes induced in cultured retinal pigment epithelium cells

Heather Dawes, Gail Mandel, Gary Matthews

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Recent electrophysiological experiments have shown that retinal pigment epithelium (RPE) cells begin to produce neuronal-type voltage-dependent sodium currents when placed in dissociated cell culture. In this study, the sodium channel types induced in cultured rat RPE cells were identified. Sodium channel mRNAs encoding two distinct alpha subunits were detected in the cultured RPE cells, brain type II/IIA, and a novel rat mRNA which we have termed RET1. These two sodium channel types may correspond to the TTX-sensitive and TTX-insensitive components of sodium current reported previously in cultured rat RPE cells.

Original language	English (US)
Pages (from-to)	1001-1005
Number of pages	5
Journal	Visual neuroscience
Volume	12
Issue number	5
DOIs	https://doi.org/10.1017/S0952523800009536
State	Published - 1995
Externally published	Yes

Keywords

Molecular biology
Retina
Retinal pigment epithelium
Sodium channel

ASJC Scopus subject areas

Physiology
Sensory Systems

Access to Document

10.1017/S0952523800009536

Cite this

@article{9cf37a7293e94ef9bbe08c5e0865cef2,

title = "Identification of sodium channel subtypes induced in cultured retinal pigment epithelium cells",

abstract = "Recent electrophysiological experiments have shown that retinal pigment epithelium (RPE) cells begin to produce neuronal-type voltage-dependent sodium currents when placed in dissociated cell culture. In this study, the sodium channel types induced in cultured rat RPE cells were identified. Sodium channel mRNAs encoding two distinct alpha subunits were detected in the cultured RPE cells, brain type II/IIA, and a novel rat mRNA which we have termed RET1. These two sodium channel types may correspond to the TTX-sensitive and TTX-insensitive components of sodium current reported previously in cultured rat RPE cells.",

keywords = "Molecular biology, Retina, Retinal pigment epithelium, Sodium channel",

author = "Heather Dawes and Gail Mandel and Gary Matthews",

note = "Funding Information: This research was supported by NIH Grants NS22518 (G. Mandel) and EYO3821 (G. Matthews). We thank Stephanie Burke for excellent technical assistance.",

year = "1995",

doi = "10.1017/S0952523800009536",

language = "English (US)",

volume = "12",

pages = "1001--1005",

journal = "Visual neuroscience",

issn = "0952-5238",

publisher = "Cambridge University Press",

number = "5",

}

TY - JOUR

T1 - Identification of sodium channel subtypes induced in cultured retinal pigment epithelium cells

AU - Dawes, Heather

AU - Mandel, Gail

AU - Matthews, Gary

N1 - Funding Information: This research was supported by NIH Grants NS22518 (G. Mandel) and EYO3821 (G. Matthews). We thank Stephanie Burke for excellent technical assistance.

PY - 1995

Y1 - 1995

N2 - Recent electrophysiological experiments have shown that retinal pigment epithelium (RPE) cells begin to produce neuronal-type voltage-dependent sodium currents when placed in dissociated cell culture. In this study, the sodium channel types induced in cultured rat RPE cells were identified. Sodium channel mRNAs encoding two distinct alpha subunits were detected in the cultured RPE cells, brain type II/IIA, and a novel rat mRNA which we have termed RET1. These two sodium channel types may correspond to the TTX-sensitive and TTX-insensitive components of sodium current reported previously in cultured rat RPE cells.

AB - Recent electrophysiological experiments have shown that retinal pigment epithelium (RPE) cells begin to produce neuronal-type voltage-dependent sodium currents when placed in dissociated cell culture. In this study, the sodium channel types induced in cultured rat RPE cells were identified. Sodium channel mRNAs encoding two distinct alpha subunits were detected in the cultured RPE cells, brain type II/IIA, and a novel rat mRNA which we have termed RET1. These two sodium channel types may correspond to the TTX-sensitive and TTX-insensitive components of sodium current reported previously in cultured rat RPE cells.

KW - Molecular biology

KW - Retina

KW - Retinal pigment epithelium

KW - Sodium channel

UR - http://www.scopus.com/inward/record.url?scp=0029360493&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029360493&partnerID=8YFLogxK

U2 - 10.1017/S0952523800009536

DO - 10.1017/S0952523800009536

M3 - Article

C2 - 8924402

AN - SCOPUS:0029360493

SN - 0952-5238

VL - 12

SP - 1001

EP - 1005

JO - Visual neuroscience

JF - Visual neuroscience

IS - 5

ER -

Identification of sodium channel subtypes induced in cultured retinal pigment epithelium cells

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this