@article{614acac142d5480790c5ccac6e3176ec,
title = "Identification of SERPINE1 as a regulator of glioblastoma cell dispersal with transcriptome profiling",
abstract = "High mortality rates of glioblastoma (GBM) patients are partly attributed to the invasive behavior of tumor cells that exhibit extensive infiltration into adjacent brain tissue, leading to rapid, inevitable, and therapy-resistant recurrence. In this study, we analyzed transcriptome of motile (dispersive) and non-motile (core) GBM cells using an in vitro spheroid dispersal model and identified SERPINE1 as a modulator of GBM cell dispersal. Genetic or pharmacological inhibition of SERPINE1 reduced spheroid dispersal and cell adhesion by regulating cell-substrate adhesion. We examined TGFβ as a potential upstream regulator of SERPINE1 expression. We also assessed the significance of SERPINE1 in GBM growth and invasion using TCGA glioma datasets and a patient-derived orthotopic GBM model. SERPINE1 expression was associated with poor prognosis and mesenchymal GBM in patients. SERPINE1 knock-down in primary GBM cells suppressed tumor growth and invasiveness in the brain. Together, our results indicate that SERPINE1 is a key player in GBM dispersal and provide insights for future anti-invasive therapy design.",
keywords = "Dispersal, GBM, Transcriptome analysis",
author = "Fidan Seker and Ahmet Cingoz and İlknur Sur-Erdem and Nazli Erguder and Alp Erkent and Fırat Uyulur and Selvan, {Myvizhi Esai} and G{\"u}m{\"u}{\c s}, {Zeynep H{\"u}lya} and Mehmet G{\"o}nen and Halil Bayraktar and Hiroaki Wakimoto and Tugba Bagci-Onder",
note = "Funding Information: Funding: Financial support was obtained from the Scientific and Technological Research Council of Turkey (TUBITAK) (315S161, T.B.-O.), and Ko{\c c} University (SF.00024, T.B.-O.). The authors gratefully acknowledge the use of services and facilities of the Ko{\c c} University Research Center for Translational Medicine (KUTTAM), funded by the Presidency of Turkey, Presidency of Strategy and Budget. The content is solely the responsibility of the authors and does not necessarily represent the official views of the Presidency of Strategy and Budget. Z.H.G. and M.E.S. thank funds from the LUNGevity foundation. M.G. was supported by the Turkish Academy of Sciences (T{\"U}BA-GEBİP; The Young Scientist Award Program) and the Science Academy of Turkey (BAGEP; The Young Scientist Award Program). Funding Information: Financial support was obtained from the Scientific and Technological Research Council of Turkey (TUBITAK) (315S161, T.B.-O.), and Ko? University (SF.00024, T.B.-O.). The authors gratefully acknowledge the use of services and facilities of the Ko? University Research Center for Translational Medicine (KUTTAM), funded by the Presidency of Turkey, Presidency of Strategy and Budget. The content is solely the responsibility of the authors and does not necessarily represent the official views of the Presidency of Strategy and Budget. Z.H.G. and M.E.S. thank funds from the LUNGevity foundation. M.G. was supported by the Turkish Academy of Sciences (T?BA-GEB?P; The Young Scientist Award Program) and the Science Academy of Turkey (BAGEP; The Young Scientist Award Program). Publisher Copyright: {\textcopyright} 2019 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2019",
month = nov,
doi = "10.3390/cancers11111651",
language = "English (US)",
volume = "11",
journal = "Cancers",
issn = "2072-6694",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "11",
}