Identification of Mycobacterium avium pathogenicity island important for macrophage and amoeba infection

Lia Danelishvili, Martin Wu, Bernadette Stang, Melanie Harriff, Stuart Cirillo, Jeffrey Cirillo, Robert Bildfell, Brian Arbogast, Luiz E. Bermudez

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

The ability to infect macrophages is a common characteristic shared among many mycobacterial species. Mycobacterium avium, Mycobacterium tuberculosis, and Mycobacterium kansasii enter macrophages, using the complement receptors CR1, CR3, CR4, and the mannose receptor. To identify M. avium genes and host cell pathways involved in the bacterial uptake by macrophages, we screened a M. avium transposon mutant library for the inability to enter macrophages. Uptake-impaired clones were selected. Sequence of six M. avium clones identified one gene involved in glycopeptidolipid biosynthesis, one gene encoding the conserved membrane protein homologue to the M. avium subsp. paratuberculosis MAP2446C gene and four others belonging to the same region of the chromosome. Analysis of the chromosome region revealed a pathogenicity island inserted between two tRNA sequences with 58% of G+C content versus 69% in the M. avium genome. The region is unique for M. avium and is not present in M. tuberculosis or M. paratuberculosis. Although the mutants did not differ from the WT bacterium regarding the binding to macrophage cell membrane, analysis of macrophage proteins after 1 h infection revealed a deficiency in the mutant to phosphorylate certain proteins on uptake. To understand M. avium interaction with two evolutionary distinct hosts, the mutants were evaluated for Acanthamoeba castellanii invasion. The defect in the ability of the mutants to invade both cells was highly similar, suggesting that M. avium might have evolved mechanisms that are used to enter amoebas and human macrophages.

Original languageEnglish (US)
Pages (from-to)11038-11043
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume104
Issue number26
DOIs
StatePublished - Jun 26 2007
Externally publishedYes

Fingerprint

Genomic Islands
Mycobacterium avium
Amoeba
Macrophages
Infection
Paratuberculosis
Mycobacterium tuberculosis
Integrin alphaXbeta2
Genes
Clone Cells
Chromosomes
Acanthamoeba castellanii
Mycobacterium kansasii
Complement Receptors
Base Composition
Transfer RNA
Membrane Proteins
Proteins
Cell Membrane
Genome

Keywords

  • Uptake

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Identification of Mycobacterium avium pathogenicity island important for macrophage and amoeba infection. / Danelishvili, Lia; Wu, Martin; Stang, Bernadette; Harriff, Melanie; Cirillo, Stuart; Cirillo, Jeffrey; Bildfell, Robert; Arbogast, Brian; Bermudez, Luiz E.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, No. 26, 26.06.2007, p. 11038-11043.

Research output: Contribution to journalArticle

Danelishvili, Lia ; Wu, Martin ; Stang, Bernadette ; Harriff, Melanie ; Cirillo, Stuart ; Cirillo, Jeffrey ; Bildfell, Robert ; Arbogast, Brian ; Bermudez, Luiz E. / Identification of Mycobacterium avium pathogenicity island important for macrophage and amoeba infection. In: Proceedings of the National Academy of Sciences of the United States of America. 2007 ; Vol. 104, No. 26. pp. 11038-11043.
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