Identification of glycoproteins in human cerebrospinal fluid with a complementary proteomic approach

Sheng Pan, Yan Wang, Joseph F. Quinn, Elaine R. Peskind, Dana Waichunas, Jake T. Wimberger, Jinghua Jin, Jane G. Li, David Zhu, Catherine Pan, Jing Zhang

Research output: Contribution to journalArticle

77 Scopus citations

Abstract

Biomarkers are pressingly needed to assist with the clinical diagnosis of neurodegenerative diseases and/or the monitoring of disease progression. Glycoproteins are enriched in bodily fluids such as human cerebrospinal fluid (CSF), an ideal source for discovering biomarkers due to its proximity to the central nervous system (CNS), and consequently can serve as diagnostic and/or therapeutic markers for CNS diseases. We report here an in-depth identification of glycoproteins in human CSF using a complementary proteomic approach which integrated hydrazide chemistry and lectin affinity column for glycoprotein enrichment, followed by multidimensional chromatography separation and tandem mass spectrometric analysis. Using stringent criteria, a total of 216 glycoproteins, including many low-abundance proteins, was identified with high confidence. Approximately one-third of these proteins was already known to be relevant to the CNS structurally or functionally. This investigation, for the first time, not only categorized many glycoproteins in human CSF but also expanded the existing overall CSF protein database.

Original languageEnglish (US)
Pages (from-to)2769-2779
Number of pages11
JournalJournal of Proteome Research
Volume5
Issue number10
DOIs
StatePublished - Oct 1 2006

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Keywords

  • Cerebrospinal fluid
  • Glycoprotein
  • Hydrazide chemistry
  • Lectin affinity column
  • Mass spectrometry
  • Proteomics

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Pan, S., Wang, Y., Quinn, J. F., Peskind, E. R., Waichunas, D., Wimberger, J. T., Jin, J., Li, J. G., Zhu, D., Pan, C., & Zhang, J. (2006). Identification of glycoproteins in human cerebrospinal fluid with a complementary proteomic approach. Journal of Proteome Research, 5(10), 2769-2779. https://doi.org/10.1021/pr060251s