Identification of ezrin as an 81-kDa tyrosine-phosphorylated protein in T cells

M. Egerton, W. H. Burgess, D. Chen, B. J. Druker, A. Bretscher, L. E. Samelson

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67 Scopus citations

Abstract

We have used APT affinity purification to isolate tyrosine-phosphorylated proteins from MRL lpr/lpr (lpr) mouse T cells. One such protein is pp81 ezrin, previously identified as a tyrosine-phosphorylated protein in epidermal growth factor-stimulated A431 carcinoma cells. Biochemical analyses in A431 and gastric parietal cells have revealed ezrin to be a cytoskeleton- associated cytosolic protein. In Jurkat T cells, however, using similar methods we have shown ezrin to be a cytosolic protein with no measurable cytoskeletal association. We also observed no increases in ezrin tyrosine phosphorylation in TCR-stimulated Jurkat T cells, unless the cells were pretreated with protein tyrosine phosphatase inhibitors, suggesting that T cell ezrin tyrosine phosphorylation is tightly controlled by protein tyrosine phosphatases. The fraction of tyrosine phosphorylated ezrin in lpr T cells was 5 to 10 times that observed in Jurkat T cells, which along with constitutive TCR-ζ phosphorylation and pp60(fyn) overexpression, is a feature of the lpr defect.

Original languageEnglish (US)
Pages (from-to)1847-1852
Number of pages6
JournalJournal of Immunology
Volume149
Issue number6
StatePublished - Jan 1 1992
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Egerton, M., Burgess, W. H., Chen, D., Druker, B. J., Bretscher, A., & Samelson, L. E. (1992). Identification of ezrin as an 81-kDa tyrosine-phosphorylated protein in T cells. Journal of Immunology, 149(6), 1847-1852.