Identification of ezrin as an 81-kDa tyrosine-phosphorylated protein in T cells

Mark Egerton, Wilson H. Burgess, Douglas Chen, Brian Druker, Anthony Bretscher, Lawrence E. Samelson

Research output: Contribution to journalArticle

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Abstract

We have used APT affinity purification to isolate tyrosine-phosphorylated proteins from MRL lpr/lpr (lpr) mouse T cells. One such protein is pp81 ezrin, previously identified as a tyrosine-phosphorylated protein in epidermal growth factor-stimulated A431 carcinoma cells. Biochemical analyses in A431 and gastric parietal cells have revealed ezrin to be a cytoskeleton-associated cytosolic protein. In Jurkat T cells, however, using similar methods we have shown ezrin to be a cytosolic protein with no measurable cytoskeletal association. We also observed no increases in ezrin tyrosine phosphorylation in TCR-stimulated Jurkat T cells, unless the cells were pretreated with protein tyrosine phosphatase inhibitors, suggesting that T cell ezrin tyrosine phosphorylation is tightly controlled by protein tyrosine phosphatases. The fraction of tyrosine phosphorylated ezrin in lpr T cells was 5 to 10 times that observed in Jurkat T cells, which along with constitutive TCR-ζ phosphorylation and pp60fyn overexpression, is a feature of the lpr defect.

Original languageEnglish (US)
Pages (from-to)1847-1852
Number of pages6
JournalJournal of Immunology
Volume149
Issue number6
StatePublished - Sep 15 1992
Externally publishedYes

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Tyrosine
T-Lymphocytes
Jurkat Cells
Proteins
Protein Tyrosine Phosphatases
Phosphorylation
Gastric Parietal Cells
Cytoskeleton
Epidermal Growth Factor
ezrin
Carcinoma

ASJC Scopus subject areas

  • Immunology

Cite this

Egerton, M., Burgess, W. H., Chen, D., Druker, B., Bretscher, A., & Samelson, L. E. (1992). Identification of ezrin as an 81-kDa tyrosine-phosphorylated protein in T cells. Journal of Immunology, 149(6), 1847-1852.

Identification of ezrin as an 81-kDa tyrosine-phosphorylated protein in T cells. / Egerton, Mark; Burgess, Wilson H.; Chen, Douglas; Druker, Brian; Bretscher, Anthony; Samelson, Lawrence E.

In: Journal of Immunology, Vol. 149, No. 6, 15.09.1992, p. 1847-1852.

Research output: Contribution to journalArticle

Egerton, M, Burgess, WH, Chen, D, Druker, B, Bretscher, A & Samelson, LE 1992, 'Identification of ezrin as an 81-kDa tyrosine-phosphorylated protein in T cells', Journal of Immunology, vol. 149, no. 6, pp. 1847-1852.
Egerton M, Burgess WH, Chen D, Druker B, Bretscher A, Samelson LE. Identification of ezrin as an 81-kDa tyrosine-phosphorylated protein in T cells. Journal of Immunology. 1992 Sep 15;149(6):1847-1852.
Egerton, Mark ; Burgess, Wilson H. ; Chen, Douglas ; Druker, Brian ; Bretscher, Anthony ; Samelson, Lawrence E. / Identification of ezrin as an 81-kDa tyrosine-phosphorylated protein in T cells. In: Journal of Immunology. 1992 ; Vol. 149, No. 6. pp. 1847-1852.
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