Identification of cGMP-Dependent protein kinase anchoring proteins (GKAPs)

Ngan Vo, Jessica M. Gettemy, Vincent M. Coghlan

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

To promote both efficiency and selectivity, many protein kinases and phosphatases are maintained in specific subcellular microenvironments through their association with anchoring proteins. In this study, we describe a new class of proteins, called GKAPS, that specifically bind the Type II cGMP-dependent protein kinase (PKG). GKAPs were detected in rat aorta, brain, and intestine using a protein overlay technique. The PKG binding proteins were distinct from AKAPs, proteins known to bind the cAMP-dependent protein kinase (PKA). Furthermore, a synthetic peptide that blocks association of PKA with AKAPs did not affect the PKG-GKAP interaction. Deletion mutagenesis was used to map the GKAP binding determinants within PKG to the N-terminal regulatory region. While most GKAPs were tissue-specific, a ubiquitous PKG-binding protein was detected and identified as myosin. Analysis of myosin fragments revealed that PKG binds within Subfragment 2. The results define a novel class of anchoring proteins that may target PKG for specific functional roles.

Original languageEnglish (US)
Pages (from-to)831-835
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume246
Issue number3
DOIs
StatePublished - May 29 1998

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Cyclic GMP-Dependent Protein Kinases
Myosins
Proteins
Protein Kinases
Cyclic GMP-Dependent Protein Kinase Type II
Carrier Proteins
Mutagenesis
Nucleic Acid Regulatory Sequences
Phosphoprotein Phosphatases
Cyclic AMP-Dependent Protein Kinases
Intestines
Aorta
Rats
Brain
Tissue
Peptides

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Identification of cGMP-Dependent protein kinase anchoring proteins (GKAPs). / Vo, Ngan; Gettemy, Jessica M.; Coghlan, Vincent M.

In: Biochemical and Biophysical Research Communications, Vol. 246, No. 3, 29.05.1998, p. 831-835.

Research output: Contribution to journalArticle

Vo, Ngan ; Gettemy, Jessica M. ; Coghlan, Vincent M. / Identification of cGMP-Dependent protein kinase anchoring proteins (GKAPs). In: Biochemical and Biophysical Research Communications. 1998 ; Vol. 246, No. 3. pp. 831-835.
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