Abstract
Candidate gene and genome-wide association studies (GWAS) have identified 15 independent genomic regions associated with bladder cancer risk. In search for additional susceptibility variants, we followed up on four promising single-nucleotide polymorphisms (SNPs) that had not achieved genome-wide significance in 6911 cases and 11 814 controls (rs6104690, rs4510656, rs5003154 and rs4907479, P < 1 × 10-6), using additional data from existing GWAS datasets and targeted genotyping for studies that did not have GWAS data. In a combined analysis, which included data on up to 15 058 cases and 286 270 controls, two SNPs achieved genome-wide statistical significance: Rs6104690 in a gene desert at 20p12.2 (P = 2.19 × 10-11) and rs4907479 within the MCF2L gene at 13q34 (P = 3.3 × 10-10). Imputation and fine-mapping analyses were performed in these two regions for a subset of 5551 bladder cancer cases and 10 242 controls. Analyses at the 13q34 region suggest a single signal marked by rs4907479. In contrast, we detected two signals in the 20p12.2 region-the first signal is marked by rs6104690, and the second signal is marked by two moderately correlated SNPs (r2 = 0.53), rs6108803 and the previously reported rs62185668. The second 20p12.2 signal is more strongly associated with the risk of muscle-invasive (T2-T4 stage) compared with non-muscle-invasive (Ta, T1 stage) bladder cancer (case-case P ≤ 0.02 for both rs62185668 and rs6108803). Functional analyses are needed to explore the biological mechanisms underlying these novel genetic associations with risk for bladder cancer. Published by Oxford University Press 2016. This work is written by (a) US Government employee(s) and is in the public domain in the US.
| Original language | English (US) |
|---|---|
| Article number | ddv492 |
| Pages (from-to) | 1203-1214 |
| Number of pages | 12 |
| Journal | Human Molecular Genetics |
| Volume | 25 |
| Issue number | 6 |
| DOIs | |
| State | Published - Aug 17 2015 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Molecular Biology
- Genetics
- Genetics(clinical)
Cite this
Identification of a novel susceptibility locus at 13q34 and refinement of the 20p12.2 region as a multi-signal locus associated with bladder cancer risk in individuals of european ancestry. / Figueroa, Jonine D.; Middlebrooks, Candace D.; Banday, A. Rouf; Ye, Yuanqing; Garcia-Closas, Montserrat; Chatterjee, Nilanjan; Koutros, Stella; Kiemeney, Lambertus A.; Rafnar, Thorunn; Bishop, Timothy; Furberg, Helena; Matullo, Giuseppe; Golka, Klaus; Gago-Dominguez, Manuela; Taylor, Jack A.; Fletcher, Tony; Siddiq, Afshan; Cortessis, Victoria K.; Kooperberg, Charles; Cussenot, Olivier; Benhamou, Simone; Prescott, Jennifer; Porru, Stefano; Dinney, Colin P.; Malats, Núria; Baris, Dalsu; Purdue, Mark P.; Jacobs, Eric J.; Albanes, Demetrius; Wang, Zhaoming; Chung, Charles C.; Vermeulen, Sita H.; Aben, Katja K.; Galesloot, Tessel E.; Thorleifsson, Gudmar; Sulem, Patrick; Stefansson, Kari; Kiltie, Anne E.; Harland, Mark; Teo, Mark; Offit, Kenneth; Vijai, Joseph; Bajorin, Dean; Kopp, Ryan; Fiorito, Giovanni; Guarrera, Simonetta; Sacerdote, Carlotta; Selinski, Silvia; Hengstler, Jan G.; Gerullis, Holger; Ovsiannikov, Daniel; Blaszkewicz, Meinolf; Castelao, Jose Esteban; Calaza, Manuel; Martinez, Maria Elena; Cordeiro, Patricia; Xu, Zongli; Panduri, Vijayalakshmi; Kumar, Rajiv; Gurzau, Eugene; Koppova, Kvetoslava; Bueno-De-Mesquita, H. Bas; Ljungberg, Börje; Clavel-Chapelon, Françoise; Weiderpass, Elisabete; Krogh, Vittorio; Dorronsoro, Miren; Travis, Ruth C.; Tjønneland, Anne; Brennan, Paul; Chang-Claude, Jenny; Riboli, Elio; Conti, David; Stern, Marianna C.; Pike, Malcolm C.; Van Den Berg, David; Yuan, Jian Min; Hohensee, Chancellor; Jeppson, Rebecca P.; Cancel-Tassin, Geraldine; Roupret, Morgan; Comperat, Eva; Turman, Constance; De Vivo, Immaculata; Giovannucci, Edward; Hunter, David J.; Kraft, Peter; Lindstrom, Sara; Carta, Angela; Pavanello, Sofia; Arici, Cecilia; Mastrangelo, Giuseppe; Kamat, Ashish M.; Zhang, Liren; Gong, Yilei; Pu, Xia; Hutchinson, Amy; Burdett, Laurie; Wheeler, William A.; Karagas, Margaret R.; Johnson, Alison; Schned, Alan; Monawar Hosain, G. M.; Schwenn, Molly; Kogevinas, Manolis; Tardón, Adonina; Serra, Consol; Carrato, Alfredo; García-Closas, Reina; Lloreta, Josep; Andriole, Gerald; Robert, Grubb; Black, Amanda; Diver, W. Ryan; Gapstur, Susan M.; Weinstein, Stephanie; Virtamo, Jarmo; Haiman, Christopher A.; Landi, Maria Teresa; Caporaso, Neil E.; Joseph, Fraumeni; Vineis, Paolo; Wu, Xifeng; Chanock, Stephen J.; Silverman, Debra T.; Prokunina-Olsson, Ludmila; Rothman, Nathaniel.
In: Human Molecular Genetics, Vol. 25, No. 6, ddv492, 17.08.2015, p. 1203-1214.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of a novel susceptibility locus at 13q34 and refinement of the 20p12.2 region as a multi-signal locus associated with bladder cancer risk in individuals of european ancestry
AU - Figueroa, Jonine D.
AU - Middlebrooks, Candace D.
AU - Banday, A. Rouf
AU - Ye, Yuanqing
AU - Garcia-Closas, Montserrat
AU - Chatterjee, Nilanjan
AU - Koutros, Stella
AU - Kiemeney, Lambertus A.
AU - Rafnar, Thorunn
AU - Bishop, Timothy
AU - Furberg, Helena
AU - Matullo, Giuseppe
AU - Golka, Klaus
AU - Gago-Dominguez, Manuela
AU - Taylor, Jack A.
AU - Fletcher, Tony
AU - Siddiq, Afshan
AU - Cortessis, Victoria K.
AU - Kooperberg, Charles
AU - Cussenot, Olivier
AU - Benhamou, Simone
AU - Prescott, Jennifer
AU - Porru, Stefano
AU - Dinney, Colin P.
AU - Malats, Núria
AU - Baris, Dalsu
AU - Purdue, Mark P.
AU - Jacobs, Eric J.
AU - Albanes, Demetrius
AU - Wang, Zhaoming
AU - Chung, Charles C.
AU - Vermeulen, Sita H.
AU - Aben, Katja K.
AU - Galesloot, Tessel E.
AU - Thorleifsson, Gudmar
AU - Sulem, Patrick
AU - Stefansson, Kari
AU - Kiltie, Anne E.
AU - Harland, Mark
AU - Teo, Mark
AU - Offit, Kenneth
AU - Vijai, Joseph
AU - Bajorin, Dean
AU - Kopp, Ryan
AU - Fiorito, Giovanni
AU - Guarrera, Simonetta
AU - Sacerdote, Carlotta
AU - Selinski, Silvia
AU - Hengstler, Jan G.
AU - Gerullis, Holger
AU - Ovsiannikov, Daniel
AU - Blaszkewicz, Meinolf
AU - Castelao, Jose Esteban
AU - Calaza, Manuel
AU - Martinez, Maria Elena
AU - Cordeiro, Patricia
AU - Xu, Zongli
AU - Panduri, Vijayalakshmi
AU - Kumar, Rajiv
AU - Gurzau, Eugene
AU - Koppova, Kvetoslava
AU - Bueno-De-Mesquita, H. Bas
AU - Ljungberg, Börje
AU - Clavel-Chapelon, Françoise
AU - Weiderpass, Elisabete
AU - Krogh, Vittorio
AU - Dorronsoro, Miren
AU - Travis, Ruth C.
AU - Tjønneland, Anne
AU - Brennan, Paul
AU - Chang-Claude, Jenny
AU - Riboli, Elio
AU - Conti, David
AU - Stern, Marianna C.
AU - Pike, Malcolm C.
AU - Van Den Berg, David
AU - Yuan, Jian Min
AU - Hohensee, Chancellor
AU - Jeppson, Rebecca P.
AU - Cancel-Tassin, Geraldine
AU - Roupret, Morgan
AU - Comperat, Eva
AU - Turman, Constance
AU - De Vivo, Immaculata
AU - Giovannucci, Edward
AU - Hunter, David J.
AU - Kraft, Peter
AU - Lindstrom, Sara
AU - Carta, Angela
AU - Pavanello, Sofia
AU - Arici, Cecilia
AU - Mastrangelo, Giuseppe
AU - Kamat, Ashish M.
AU - Zhang, Liren
AU - Gong, Yilei
AU - Pu, Xia
AU - Hutchinson, Amy
AU - Burdett, Laurie
AU - Wheeler, William A.
AU - Karagas, Margaret R.
AU - Johnson, Alison
AU - Schned, Alan
AU - Monawar Hosain, G. M.
AU - Schwenn, Molly
AU - Kogevinas, Manolis
AU - Tardón, Adonina
AU - Serra, Consol
AU - Carrato, Alfredo
AU - García-Closas, Reina
AU - Lloreta, Josep
AU - Andriole, Gerald
AU - Robert, Grubb
AU - Black, Amanda
AU - Diver, W. Ryan
AU - Gapstur, Susan M.
AU - Weinstein, Stephanie
AU - Virtamo, Jarmo
AU - Haiman, Christopher A.
AU - Landi, Maria Teresa
AU - Caporaso, Neil E.
AU - Joseph, Fraumeni
AU - Vineis, Paolo
AU - Wu, Xifeng
AU - Chanock, Stephen J.
AU - Silverman, Debra T.
AU - Prokunina-Olsson, Ludmila
AU - Rothman, Nathaniel
PY - 2015/8/17
Y1 - 2015/8/17
N2 - Candidate gene and genome-wide association studies (GWAS) have identified 15 independent genomic regions associated with bladder cancer risk. In search for additional susceptibility variants, we followed up on four promising single-nucleotide polymorphisms (SNPs) that had not achieved genome-wide significance in 6911 cases and 11 814 controls (rs6104690, rs4510656, rs5003154 and rs4907479, P < 1 × 10-6), using additional data from existing GWAS datasets and targeted genotyping for studies that did not have GWAS data. In a combined analysis, which included data on up to 15 058 cases and 286 270 controls, two SNPs achieved genome-wide statistical significance: Rs6104690 in a gene desert at 20p12.2 (P = 2.19 × 10-11) and rs4907479 within the MCF2L gene at 13q34 (P = 3.3 × 10-10). Imputation and fine-mapping analyses were performed in these two regions for a subset of 5551 bladder cancer cases and 10 242 controls. Analyses at the 13q34 region suggest a single signal marked by rs4907479. In contrast, we detected two signals in the 20p12.2 region-the first signal is marked by rs6104690, and the second signal is marked by two moderately correlated SNPs (r2 = 0.53), rs6108803 and the previously reported rs62185668. The second 20p12.2 signal is more strongly associated with the risk of muscle-invasive (T2-T4 stage) compared with non-muscle-invasive (Ta, T1 stage) bladder cancer (case-case P ≤ 0.02 for both rs62185668 and rs6108803). Functional analyses are needed to explore the biological mechanisms underlying these novel genetic associations with risk for bladder cancer. Published by Oxford University Press 2016. This work is written by (a) US Government employee(s) and is in the public domain in the US.
AB - Candidate gene and genome-wide association studies (GWAS) have identified 15 independent genomic regions associated with bladder cancer risk. In search for additional susceptibility variants, we followed up on four promising single-nucleotide polymorphisms (SNPs) that had not achieved genome-wide significance in 6911 cases and 11 814 controls (rs6104690, rs4510656, rs5003154 and rs4907479, P < 1 × 10-6), using additional data from existing GWAS datasets and targeted genotyping for studies that did not have GWAS data. In a combined analysis, which included data on up to 15 058 cases and 286 270 controls, two SNPs achieved genome-wide statistical significance: Rs6104690 in a gene desert at 20p12.2 (P = 2.19 × 10-11) and rs4907479 within the MCF2L gene at 13q34 (P = 3.3 × 10-10). Imputation and fine-mapping analyses were performed in these two regions for a subset of 5551 bladder cancer cases and 10 242 controls. Analyses at the 13q34 region suggest a single signal marked by rs4907479. In contrast, we detected two signals in the 20p12.2 region-the first signal is marked by rs6104690, and the second signal is marked by two moderately correlated SNPs (r2 = 0.53), rs6108803 and the previously reported rs62185668. The second 20p12.2 signal is more strongly associated with the risk of muscle-invasive (T2-T4 stage) compared with non-muscle-invasive (Ta, T1 stage) bladder cancer (case-case P ≤ 0.02 for both rs62185668 and rs6108803). Functional analyses are needed to explore the biological mechanisms underlying these novel genetic associations with risk for bladder cancer. Published by Oxford University Press 2016. This work is written by (a) US Government employee(s) and is in the public domain in the US.
UR - http://www.scopus.com/inward/record.url?scp=84960105525&partnerID=8YFLogxK
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U2 - 10.1093/hmg/ddv492
DO - 10.1093/hmg/ddv492
M3 - Article
VL - 25
SP - 1203
EP - 1214
JO - Human Molecular Genetics
JF - Human Molecular Genetics
SN - 0964-6906
IS - 6
M1 - ddv492
ER -