Identification and characterization of human cytomegalovirus-encoded microRNAs

Finn Grey, Andy Antoniewicz, Edwards Allen, Julie Saugstad, Andy McShea, James C. Carrington, Jay Nelson

Research output: Contribution to journalArticle

209 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are an extensive class of noncoding genes that regulate gene expression through posttranscriptional repression. Given the potential for large viral genomes to encode these transcripts, we examined the human cytomegalovirus AD169 genome for miRNAs using a bioinformatics approach. We identified 406 potential stem-loops, of which 110 were conserved between chimpanzee cytomegalovirus and several strains of human cytomegalovirus. Of these conserved stem-loops, 13 exhibited a significant score using the MiRscan algorithm. Examination of total RNA from human cytomegalovirus-infected cells demonstrated that 5 of the 13 predicted miRNAs were expressed during infection. These studies demonstrate that human cytomegalovirus encodes multiple conserved miRNAs and suggest that human cytomegalovirus may utilize an miRNA strategy to regulate cellular and viral gene function.

Original languageEnglish (US)
Pages (from-to)12095-12099
Number of pages5
JournalJournal of Virology
Volume79
Issue number18
DOIs
StatePublished - Sep 2005

Fingerprint

Human herpesvirus 5
Forensic Anthropology
Cytomegalovirus
MicroRNAs
microRNA
stems
genome
Pan troglodytes
Viral Genes
bioinformatics
Viral Genome
Computational Biology
genes
RNA
gene expression
Genome
Gene Expression
infection
Infection
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Identification and characterization of human cytomegalovirus-encoded microRNAs. / Grey, Finn; Antoniewicz, Andy; Allen, Edwards; Saugstad, Julie; McShea, Andy; Carrington, James C.; Nelson, Jay.

In: Journal of Virology, Vol. 79, No. 18, 09.2005, p. 12095-12099.

Research output: Contribution to journalArticle

Grey, Finn ; Antoniewicz, Andy ; Allen, Edwards ; Saugstad, Julie ; McShea, Andy ; Carrington, James C. ; Nelson, Jay. / Identification and characterization of human cytomegalovirus-encoded microRNAs. In: Journal of Virology. 2005 ; Vol. 79, No. 18. pp. 12095-12099.
@article{ad47613bc53b40a6b023bf06caaa1921,
title = "Identification and characterization of human cytomegalovirus-encoded microRNAs",
abstract = "MicroRNAs (miRNAs) are an extensive class of noncoding genes that regulate gene expression through posttranscriptional repression. Given the potential for large viral genomes to encode these transcripts, we examined the human cytomegalovirus AD169 genome for miRNAs using a bioinformatics approach. We identified 406 potential stem-loops, of which 110 were conserved between chimpanzee cytomegalovirus and several strains of human cytomegalovirus. Of these conserved stem-loops, 13 exhibited a significant score using the MiRscan algorithm. Examination of total RNA from human cytomegalovirus-infected cells demonstrated that 5 of the 13 predicted miRNAs were expressed during infection. These studies demonstrate that human cytomegalovirus encodes multiple conserved miRNAs and suggest that human cytomegalovirus may utilize an miRNA strategy to regulate cellular and viral gene function.",
author = "Finn Grey and Andy Antoniewicz and Edwards Allen and Julie Saugstad and Andy McShea and Carrington, {James C.} and Jay Nelson",
year = "2005",
month = "9",
doi = "10.1128/JVI.79.18.12095-12099.2005",
language = "English (US)",
volume = "79",
pages = "12095--12099",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "18",

}

TY - JOUR

T1 - Identification and characterization of human cytomegalovirus-encoded microRNAs

AU - Grey, Finn

AU - Antoniewicz, Andy

AU - Allen, Edwards

AU - Saugstad, Julie

AU - McShea, Andy

AU - Carrington, James C.

AU - Nelson, Jay

PY - 2005/9

Y1 - 2005/9

N2 - MicroRNAs (miRNAs) are an extensive class of noncoding genes that regulate gene expression through posttranscriptional repression. Given the potential for large viral genomes to encode these transcripts, we examined the human cytomegalovirus AD169 genome for miRNAs using a bioinformatics approach. We identified 406 potential stem-loops, of which 110 were conserved between chimpanzee cytomegalovirus and several strains of human cytomegalovirus. Of these conserved stem-loops, 13 exhibited a significant score using the MiRscan algorithm. Examination of total RNA from human cytomegalovirus-infected cells demonstrated that 5 of the 13 predicted miRNAs were expressed during infection. These studies demonstrate that human cytomegalovirus encodes multiple conserved miRNAs and suggest that human cytomegalovirus may utilize an miRNA strategy to regulate cellular and viral gene function.

AB - MicroRNAs (miRNAs) are an extensive class of noncoding genes that regulate gene expression through posttranscriptional repression. Given the potential for large viral genomes to encode these transcripts, we examined the human cytomegalovirus AD169 genome for miRNAs using a bioinformatics approach. We identified 406 potential stem-loops, of which 110 were conserved between chimpanzee cytomegalovirus and several strains of human cytomegalovirus. Of these conserved stem-loops, 13 exhibited a significant score using the MiRscan algorithm. Examination of total RNA from human cytomegalovirus-infected cells demonstrated that 5 of the 13 predicted miRNAs were expressed during infection. These studies demonstrate that human cytomegalovirus encodes multiple conserved miRNAs and suggest that human cytomegalovirus may utilize an miRNA strategy to regulate cellular and viral gene function.

UR - http://www.scopus.com/inward/record.url?scp=24644484014&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=24644484014&partnerID=8YFLogxK

U2 - 10.1128/JVI.79.18.12095-12099.2005

DO - 10.1128/JVI.79.18.12095-12099.2005

M3 - Article

VL - 79

SP - 12095

EP - 12099

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 18

ER -