Identification and characteristic of LDL-subfractions in human plasma.

Y. D. Kholodova, William Harris

Research output: Contribution to journalArticle

Abstract

Density gradient ultracentrifugation and high resolution polyacrylamide gel electrophoresis (Lipoprint LDL-system) were used to examine the discrete LDL subfraction patterns in normal and hyperlipidemic patients. Two new programs for LDL and VLDL subfractions were elaborated and a new method of evaluation for cholesterol content in LDL-subfractions, based on area distribution ratio of densitograms without density gradient ultracentrifugation, was created. The predominance of cholesterol and triglycerides in dense LDL3-subclass (d-1,048-1,063 g/ml) under hypercholesterolemia and hypertriglyceridemia was found. Lipoprint LDL-electrophoresis method was developed for the characteristic of lipemic state of organism and detection and quantitation of plasma LDL subfractions.

Original languageEnglish (US)
Pages (from-to)60-65
Number of pages6
JournalUkrainskii biokhimicheskii zhurnal
Volume67
Issue number6
StatePublished - Nov 1995
Externally publishedYes

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Plasma (human)
Ultracentrifugation
Electrophoresis
Cholesterol
Hypertriglyceridemia
Hypercholesterolemia
Polyacrylamide Gel Electrophoresis
Triglycerides
low density lipoprotein inhibitor
oxidized low density lipoprotein
Plasmas

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Identification and characteristic of LDL-subfractions in human plasma. / Kholodova, Y. D.; Harris, William.

In: Ukrainskii biokhimicheskii zhurnal, Vol. 67, No. 6, 11.1995, p. 60-65.

Research output: Contribution to journalArticle

Kholodova, Y. D. ; Harris, William. / Identification and characteristic of LDL-subfractions in human plasma. In: Ukrainskii biokhimicheskii zhurnal. 1995 ; Vol. 67, No. 6. pp. 60-65.
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