Hypothalamic proopiomelanocortin neurons are glucose responsive and express KATP channels

Nurhadi Ibrahim, Martha A. Bosch, James L. Smart, Jian Qiu, Marcelo Rubinstein, Oline Ronnekleiv, Malcolm J. Low, Martin Kelly

Research output: Contribution to journalArticle

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Abstract

Hypothalamic proopiomelanocortin (POMC) neurons are critical for controlling homeostatic functions in the mammal. We used a transgenic mouse model in which the POMC neurons were labeled with enhanced green fluorescent protein to perform visualized, whole-cell patch recordings from prepubertal female hypothalamic slices. The mouse POMC-enhanced green fluorescent protein neurons expressed the same endogenous conductances (a transient outward K+ current and a hyperpolarization-activated, cation current) that have been described for guinea pig POMC neurons. In addition, the selective μ-opioid receptor agonist DAMGO induced an outward current (maximum of 12.8 ± 1.2 pA), which reversed at K+ equilibrium potential (EK+), in the majority (85%) of POMC neurons with an EC50 of 102 nM. This response was blocked by the opioid receptor antagonist naloxone with an inhibition constant of 3.1 nM. In addition, the γ-aminobutyric acidB receptor agonist baclofen (40 μM) caused an outward current (21.6 ± 4.0 pA) that reversed at EK+ in these same neurons. The ATP-sensitive potassium channel opener diazoxide also induced an outward K+ current (maximum of 18.7 ± 2.2 pA) in the majority (92%) of POMC neurons with an EC50 of 61 μM. The response to diazoxide was blocked by the sulfonylurea tolbutamide, indicating that the POMC neurons express both Kir6.2 and sulfonylurea receptor 1 channel subunits, which was verified using single cell RT-PCR. This pharmacological and molecular profile suggested that POMC neurons might be sensitive to metabolic inhibition, and indeed, we found that their firing rate varied with changes in glucose concentrations. Therefore, it appears that POMC neurons may function as an integrator of metabolic cues and synaptic input for controlling homeostasis in the mammal.

Original languageEnglish (US)
Pages (from-to)1331-1340
Number of pages10
JournalEndocrinology
Volume144
Issue number4
DOIs
StatePublished - Apr 1 2003

Fingerprint

KATP Channels
Pro-Opiomelanocortin
Neurons
Glucose
Diazoxide
Mammals
Sulfonylurea Receptors
Ala(2)-MePhe(4)-Gly(5)-enkephalin
GABA-B Receptors
Tolbutamide
Narcotic Antagonists
Opioid Receptors
Patch-Clamp Techniques
Naloxone
Transgenic Mice
Cues
Cations
Guinea Pigs
Homeostasis
Pharmacology

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Hypothalamic proopiomelanocortin neurons are glucose responsive and express KATP channels. / Ibrahim, Nurhadi; Bosch, Martha A.; Smart, James L.; Qiu, Jian; Rubinstein, Marcelo; Ronnekleiv, Oline; Low, Malcolm J.; Kelly, Martin.

In: Endocrinology, Vol. 144, No. 4, 01.04.2003, p. 1331-1340.

Research output: Contribution to journalArticle

Ibrahim, Nurhadi ; Bosch, Martha A. ; Smart, James L. ; Qiu, Jian ; Rubinstein, Marcelo ; Ronnekleiv, Oline ; Low, Malcolm J. ; Kelly, Martin. / Hypothalamic proopiomelanocortin neurons are glucose responsive and express KATP channels. In: Endocrinology. 2003 ; Vol. 144, No. 4. pp. 1331-1340.
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