Hypervolemia of pregnancy is not maintained in mice chronically overexpressing angiotensinogen

Terry K. Morgan, Andreas Rohrwasser, Ling Zhao, Elaine Hillas, Tong Cheng, Kenneth J. Ward, Jean Marc Lalouel

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Objective: Women who develop pre-eclampsia show significantly less hypervolemia of pregnancy, compared with controls. We have shown that chronically elevated angiotensinogen expression increases a woman's risk of developing pre-eclampsia. Our objective was to determine whether increased angiotensinogen expression is sufficient to cause failed hypervolemia. Study design: To isolate the effects of elevated angiotensinogen expression, we studied transgenic mice with either 2 or 3 copies of the murine angiotensinogen gene. Plasma volume was measured by Evans blue dye dilution, and kidney sections were immunostained for angiotensinogen and renin. Results: Three-copy mice failed to maintain hypervolemia after midgestation (P < .01) and failed to up-regulate renin expression in the distal nephron, compared with 2-copy controls. Intrarenal angiotensinogen was up-regulated during pregnancy in both genotypes. Conclusion: Chronically elevated angiotensinogen expression is sufficient to cause failed hypervolemia of pregnancy. Whether this observation is related to failed up-regulation of distal tubule renin expression requires further study.

Original languageEnglish (US)
Pages (from-to)1700-1706
Number of pages7
JournalAmerican journal of obstetrics and gynecology
Volume195
Issue number6
DOIs
StatePublished - Dec 1 2006

Keywords

  • Intrarenal renin-angiotensin system
  • Kidney
  • Mouse
  • Plasma volume
  • Pregnancy

ASJC Scopus subject areas

  • Obstetrics and Gynecology

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