Hypertonic saline resuscitates dogs in endotoxin shock

Richard Mullins, Russell W. Hudgens

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In this experiment we determined if infusions of hypertonic saline (HS, 1080 Na meq/liter) could resuscitate dogs in endotoxin shock as effectively as Ringer's lactate (RL, 130 Na meq/liter). Anesthetized dogs received iv 0.5 mg/kg of Escherichia coli endotoxin, and mean arterial pressure (MAP, mm Hg) decreased from 148 ± 5 to 58 ± 14 within 30 min. To resuscitate the dogs 13 meq/kg of sodium was intravenously infused over 90 min as either a 10% body weight load of RL (n = 5) or a 1.2% body weight load of HS (n = 5). Both solutions produced an equivalent hemodynamic resuscitation 3 to 4 hr postinfusion with an increase in MAP (RL, 119 ± 4; HS, 108 ± 7), the restoration of cardiac outputs to baseline (RL, 2.0 ± 0.2; HS, 1.9 ± 0.3 liter/min), and similar renal inulin clearances (RL, 48 ± 16; HS, 44 ± 7 ml/min). The net fluid gain (resuscitation fluid volume infused minus urine output as percentage of body weight) was much greater in the RL group (7.2 ± 1.0%) than in the HS group (0.48 ± 0.2%). Plasma volume (PV, percentage of body weight) was measured with Evans blue dye in these splenectomized dogs. The increase in PV in the RL dogs (1.25 ± 0.04%) was slightly greater than the increase in the HS group (0.94 ± 0.13). Prenodal skin lymph was collected from both hindpaws, and the fractional increase in skin lymph flow after RL (4.5 ± 2.9) was greater than the increase in the HS group (1.7 ± 0.3). A small volume of HS can resuscitate dogs in endotoxin shock as well as an equal sodium load of RL by expanding the plasma volume with fluid that shifts to the intravascular compartment.

Original languageEnglish (US)
Pages (from-to)37-44
Number of pages8
JournalJournal of Surgical Research
Volume43
Issue number1
DOIs
StatePublished - 1987
Externally publishedYes

Fingerprint

Endotoxins
Shock
Dogs
Body Weight
Plasma Volume
Lymph
Resuscitation
Sodium
Fluid Shifts
Evans Blue
Skin
Inulin
Cardiac Output
Arterial Pressure
Coloring Agents
Hemodynamics
Urine
Kidney

ASJC Scopus subject areas

  • Surgery

Cite this

Hypertonic saline resuscitates dogs in endotoxin shock. / Mullins, Richard; Hudgens, Russell W.

In: Journal of Surgical Research, Vol. 43, No. 1, 1987, p. 37-44.

Research output: Contribution to journalArticle

Mullins, Richard ; Hudgens, Russell W. / Hypertonic saline resuscitates dogs in endotoxin shock. In: Journal of Surgical Research. 1987 ; Vol. 43, No. 1. pp. 37-44.
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abstract = "In this experiment we determined if infusions of hypertonic saline (HS, 1080 Na meq/liter) could resuscitate dogs in endotoxin shock as effectively as Ringer's lactate (RL, 130 Na meq/liter). Anesthetized dogs received iv 0.5 mg/kg of Escherichia coli endotoxin, and mean arterial pressure (MAP, mm Hg) decreased from 148 ± 5 to 58 ± 14 within 30 min. To resuscitate the dogs 13 meq/kg of sodium was intravenously infused over 90 min as either a 10{\%} body weight load of RL (n = 5) or a 1.2{\%} body weight load of HS (n = 5). Both solutions produced an equivalent hemodynamic resuscitation 3 to 4 hr postinfusion with an increase in MAP (RL, 119 ± 4; HS, 108 ± 7), the restoration of cardiac outputs to baseline (RL, 2.0 ± 0.2; HS, 1.9 ± 0.3 liter/min), and similar renal inulin clearances (RL, 48 ± 16; HS, 44 ± 7 ml/min). The net fluid gain (resuscitation fluid volume infused minus urine output as percentage of body weight) was much greater in the RL group (7.2 ± 1.0{\%}) than in the HS group (0.48 ± 0.2{\%}). Plasma volume (PV, percentage of body weight) was measured with Evans blue dye in these splenectomized dogs. The increase in PV in the RL dogs (1.25 ± 0.04{\%}) was slightly greater than the increase in the HS group (0.94 ± 0.13). Prenodal skin lymph was collected from both hindpaws, and the fractional increase in skin lymph flow after RL (4.5 ± 2.9) was greater than the increase in the HS group (1.7 ± 0.3). A small volume of HS can resuscitate dogs in endotoxin shock as well as an equal sodium load of RL by expanding the plasma volume with fluid that shifts to the intravascular compartment.",
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