Hyperpolarization of hypothalamic parvocellular neurons by 17 β-estradiol and their identification through intracellular staining with procion yellow

Martin Kelly, U. Kuhnt, W. Wuttke

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Intracellular recordings and injections of procion yellow (PY) were made in parvocellular neurons in hypothalamic slices of female guinea pigs. Eighty-five neurons, with an average resting membrane potential of -35 mV, were recorded in the arcuate (ARC) ventromedial (VM), and in the cellpoor zones between the ARC and VM. Eleven of the ARC neurons and four neurons from the cell-poor zone could be driven antidromically by median eminence (ME) stimulation, nine other neurons from the three areas could be driven orthodromically by stria terminalis (ST) stimulation. Twenty-eight parvocellular neurons were tested with 17 β-estradiol (E2), which was applied in the bathing medium as the free steroid. Eleven neurons (nine ARC and two cell-poor-zone neurons) were hyperpolarized 2 to 24 mV by 10-10 M E2 concentrations. 10-8 M estrone concentration was without effect on three of these cells. Through the intracellular injection of PY, the estrogen-sensitive neurons (N = 11) were identified as small fusiform cells with few dendrites. Spine-like appendages were found on only one of these cells. None of the larger pyramidal-like neurons of these areas responded to the application of E2.

Original languageEnglish (US)
Pages (from-to)440-447
Number of pages8
JournalExperimental Brain Research
Volume40
Issue number4
DOIs
StatePublished - Nov 1980
Externally publishedYes

Fingerprint

Estradiol
Staining and Labeling
Neurons
procion yellow
Median Eminence
Injections
Estrone
Pyramidal Cells
Dendrites
Membrane Potentials
Estrogens
Guinea Pigs
Spine
Steroids

Keywords

  • Estrogen
  • Humoral control
  • Intracellular recordings
  • Parvocellular neurons
  • Procion yellow

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

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abstract = "Intracellular recordings and injections of procion yellow (PY) were made in parvocellular neurons in hypothalamic slices of female guinea pigs. Eighty-five neurons, with an average resting membrane potential of -35 mV, were recorded in the arcuate (ARC) ventromedial (VM), and in the cellpoor zones between the ARC and VM. Eleven of the ARC neurons and four neurons from the cell-poor zone could be driven antidromically by median eminence (ME) stimulation, nine other neurons from the three areas could be driven orthodromically by stria terminalis (ST) stimulation. Twenty-eight parvocellular neurons were tested with 17 β-estradiol (E2), which was applied in the bathing medium as the free steroid. Eleven neurons (nine ARC and two cell-poor-zone neurons) were hyperpolarized 2 to 24 mV by 10-10 M E2 concentrations. 10-8 M estrone concentration was without effect on three of these cells. Through the intracellular injection of PY, the estrogen-sensitive neurons (N = 11) were identified as small fusiform cells with few dendrites. Spine-like appendages were found on only one of these cells. None of the larger pyramidal-like neurons of these areas responded to the application of E2.",
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AU - Wuttke, W.

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