Hypercapnic blood flow reactivity not increased by α-blockade or cordotomy in piglets

L. E. Moore, Jeffrey Kirsch, M. A. Helfaer, R. S. Greenberg, R. J. Traystman

Research output: Contribution to journalArticle

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Abstract

We tested the hypothesis that differential sympathetic innervation explains the attenuated cerebral blood flow (CBF) response to hypercapnia (hyper) in forebrain (fb) compared with brain stem in 1- to 2-wk-old piglets. In pentobarbital sodium-anesthetized piglets, CBF (microspheres) was measured during hypocapnia, normocapnia (normo), and hypercapnia [arterial CO2 partial pressure (Pa(CO)2) of 25, 40, and 65 mmHg, respectively] in random sequence. After pretreatment values were obtained, piglets were randomized to undergo sham treatment (n = 5), high cervical spinal cord transection (n = 6), or pharmacological α-adrenergic blockade (prazosin 1 mg/kg + yohimbine 1 mg/kg, n = 6). After each experimental treatment, CO2 reactivity was again measured. Before experimental manipulation, hypercapnic reactivity [(CBF(hyper) - CBF(normo))/(Pa(CO2hyper) - Pa(CO2normo))] in brain stem was approximately three times greater than in forebrain (e.g., sham; 3.6 ± 0.8 vs. 1.2 ± 0.3 ml · min-1 · 100 g-1 · mmHg-1). Hypercapnic reactivity in forebrain was not increased by cord transection (1.4 ± 0.3 vs. 1.1 ± 0.2 ml · min-1 · 100 g-1 mmHg-1) or α-blockade (1.6 ± 0.6 vs. 1.2 ± 0.4 ml · min-1 · 100 g-1 · mmHg-1). Likewise, hypercapnic cerebral vascular resistance (CVR) was unchanged by experimental treatment (e.g., CVR(fb); cord transection 1.1 ± 0.1 vs. 1.0 ± 0.1; α-blockade 1.1 ± 0.2 vs. 1.0 ± 0.1 mmHg · ml-1 · min-1 · 100 g-1). Hypocapnic vasoconstriction, however, was attenuated by both cord transection and α- blockade in forebrain and brain stem. We conclude that physiological stimulation of the noradrenergic component of the sympathetic nervous system does not explain regional differences in CBF reactivity during hypercapnia in 1- to 2-wk-old piglets. The sympathetic nervous system, however, may participate in hypocapnic vasoconstriction.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume262
Issue number6 31-6
StatePublished - 1992
Externally publishedYes

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Cerebrovascular Circulation
Cordotomy
Prosencephalon
Hypercapnia
Brain Stem
Sympathetic Nervous System
Vasoconstriction
Vascular Resistance
Hypocapnia
Yohimbine
Partial Pressure
Prazosin
Pentobarbital
Carbon Monoxide
Spinal Cord Injuries
Microspheres
Adrenergic Agents
Placebos
Pharmacology
Therapeutics

Keywords

  • cerebral blood flow
  • hypercapnia
  • newborns
  • sympathetic innervation

ASJC Scopus subject areas

  • Physiology

Cite this

Hypercapnic blood flow reactivity not increased by α-blockade or cordotomy in piglets. / Moore, L. E.; Kirsch, Jeffrey; Helfaer, M. A.; Greenberg, R. S.; Traystman, R. J.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 262, No. 6 31-6, 1992.

Research output: Contribution to journalArticle

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