Hyaluronan anchored to activated CD44 on central nervous system vascular endothelial cells promotes lymphocyte extravasation in experimental autoimmune encephalomyelitis

Clayton W. Winkler, Scott C. Foster, Steven Matsumoto, Marnie A. Preston, Rubing Xing, Bruce F. Bebo, Fatima Banine, Michelle A. Berny-Lang, Asako Itakura, Owen McCarty, Lawrence (Larry) Sherman

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The extravasation of lymphocytes across central nervous system (CNS) vascular endothelium is a key step in inflammatory demyelinating diseases including multiple sclerosis (MS) and experimental autoimmune encephalomyelitis (EAE). The glycosaminoglycan hyaluronan (HA) and its receptor, CD44, have been implicated in this process but their precise roles are unclear. We find that CD44-/- mice have a delayed onset of EAE compared with wild type animals. Using an in vitro lymphocyte rolling assay, we find that fewer slow rolling (-/- brain vascular endothelial cells (ECs) than with WT ECs. We also find that CD44-/- ECs fail to anchor HA to their surfaces, and that slow rolling lymphocyte interactions with WT ECs are inhibited when the ECs are treated with a pegylated form of the PH20 hyaluronidase (PEG-PH20). Subcutaneous injection of PEG-PH20 delays the onset of EAE symptoms by ∼1 day and transiently ameliorates symptoms for 2 days following disease onset. These improved symptoms correspond histologically to degradation of HA in the lumen of CNS blood vessels, decreased demyelination, and impaired CD4 + T-cell extravasation. Collectively these data suggest that HA tethered to CD44 on CNS ECs is critical for the extravasation of activated T cells into the CNS providing new insight into the mechanisms promoting inflammatory demyelinating disease.

Original languageEnglish (US)
Pages (from-to)33237-33251
Number of pages15
JournalJournal of Biological Chemistry
Volume287
Issue number40
DOIs
StatePublished - Sep 28 2012

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Autoimmune Experimental Encephalomyelitis
Lymphocytes
Endothelial cells
Neurology
Hyaluronic Acid
Central Nervous System
Endothelial Cells
Demyelinating Diseases
T-cells
Polyethylene glycols
CD44 Antigens
T-Lymphocytes
Wild Animals
Hyaluronoglucosaminidase
Vascular Endothelium
Blood vessels
Subcutaneous Injections
Glycosaminoglycans
Anchors
Multiple Sclerosis

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Hyaluronan anchored to activated CD44 on central nervous system vascular endothelial cells promotes lymphocyte extravasation in experimental autoimmune encephalomyelitis. / Winkler, Clayton W.; Foster, Scott C.; Matsumoto, Steven; Preston, Marnie A.; Xing, Rubing; Bebo, Bruce F.; Banine, Fatima; Berny-Lang, Michelle A.; Itakura, Asako; McCarty, Owen; Sherman, Lawrence (Larry).

In: Journal of Biological Chemistry, Vol. 287, No. 40, 28.09.2012, p. 33237-33251.

Research output: Contribution to journalArticle

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