Hunan growth hormone induces and maintains c-myc gene expression in nd2 lymphoma cells

W. H. Fleming, P. R. Murphy, L. J. Murphy, T. W. Hatton, R. J. Matusik, H. G. Friesen

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

The effect of human GH (hGH) on c-myc gene expression and cell proliferation in the Nb2 lymphoma cell line was examined. The addition of hormone to stationary cultures of Nb2 cells resulted in an increased accumulation of c-myc transcripts that was detectable within 15 min, reached a maximum induction of 25-fold in 3 h, and then gradually declined. Although maximal accumulation of c-myc transcripts occurred within 3 h after hGH addition, removal of the hormone even after 4 h of treatment resulted in a failure of the cells to proliferate. This loss of proliferative capacity following hormone removal was accompanied by a 20-fold reduction in the level of c-myc transcripts. These results indicate that hGH induced proliferation of Nb2 cells is associated with the induction and maintenance of c-myc gene expression.

Original languageEnglish (US)
Pages (from-to)2547-2549
Number of pages3
JournalEndocrinology
Volume117
Issue number6
DOIs
StatePublished - Jan 1 1985

    Fingerprint

ASJC Scopus subject areas

  • Endocrinology

Cite this

Fleming, W. H., Murphy, P. R., Murphy, L. J., Hatton, T. W., Matusik, R. J., & Friesen, H. G. (1985). Hunan growth hormone induces and maintains c-myc gene expression in nd2 lymphoma cells. Endocrinology, 117(6), 2547-2549. https://doi.org/10.1210/endo-117-6-2547