Human transfer factor: fractionation by electrofocusing and high pressure, reverse phase chromatography

A. A. Vandenbark, D. R. Burger, D. L. Dreyer, G. D. Daves, R. M. Vetto

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The biologically active fraction of human transfer factor (Sephadex G 25, fraction III) has been separated by exclusion chromatography into two component fractions (IIIa and IIIb). Fraction IIIa transferred donor reactivities to PPD or KLH into 9 of 9 recipients. Fraction IIIb did not transfer donor reactivities (0 of 8 transfers) but did not produce both antigen independent and antigen dependent dermal reactions in recipients. Fractionation by electrofocusing and high pressure reverse phase chromatography revealed that IIIa has a major UV 254 absorbing component and that fraction IIIb has at least three major and three minor UV absorbing components. The major component of IIIa was isolated by preparative electrofocusing, had an isoelectric point of 1.6, and transferred KLH reactivity to four of five recipients.

Original languageEnglish (US)
Pages (from-to)636-641
Number of pages6
JournalJournal of Immunology
Volume118
Issue number2
StatePublished - Oct 3 1977

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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