Human transfer factor

fractionation by electrofocusing and high pressure, reverse phase chromatography

Arthur Vandenbark, D. R. Burger, D. L. Dreyer, G. D. Daves, R. M. Vetto

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The biologically active fraction of human transfer factor (Sephadex G 25, fraction III) has been separated by exclusion chromatography into two component fractions (IIIa and IIIb). Fraction IIIa transferred donor reactivities to PPD or KLH into 9 of 9 recipients. Fraction IIIb did not transfer donor reactivities (0 of 8 transfers) but did not produce both antigen independent and antigen dependent dermal reactions in recipients. Fractionation by electrofocusing and high pressure reverse phase chromatography revealed that IIIa has a major UV 254 absorbing component and that fraction IIIb has at least three major and three minor UV absorbing components. The major component of IIIa was isolated by preparative electrofocusing, had an isoelectric point of 1.6, and transferred KLH reactivity to four of five recipients.

Original languageEnglish (US)
Pages (from-to)636-641
Number of pages6
JournalJournal of Immunology
Volume118
Issue number2
StatePublished - 1977
Externally publishedYes

Fingerprint

Transfer Factor
Isoelectric Focusing
Reverse-Phase Chromatography
Antigens
Pressure
Tuberculin
Isoelectric Point
Gel Chromatography
Skin
sephadex

ASJC Scopus subject areas

  • Immunology

Cite this

Human transfer factor : fractionation by electrofocusing and high pressure, reverse phase chromatography. / Vandenbark, Arthur; Burger, D. R.; Dreyer, D. L.; Daves, G. D.; Vetto, R. M.

In: Journal of Immunology, Vol. 118, No. 2, 1977, p. 636-641.

Research output: Contribution to journalArticle

Vandenbark, Arthur ; Burger, D. R. ; Dreyer, D. L. ; Daves, G. D. ; Vetto, R. M. / Human transfer factor : fractionation by electrofocusing and high pressure, reverse phase chromatography. In: Journal of Immunology. 1977 ; Vol. 118, No. 2. pp. 636-641.
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