Human Neonatal Dendritic Cells Are Competent in MHC Class I Antigen Processing and Presentation

Marielle Gold, Tammie L. Robinson, Matthew S. Cook, Laura K. Byrd, Heather D. Ehlinger, David Lewinsohn, Deborah Lewinsohn

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Neonates are clearly more susceptible to severe disease following infection with a variety of pathogens than are adults. However, the causes for this are unclear and are often attributed to immunological immaturity. While several aspects of immunity differ between adults and neonates, the capacity of dendritic cells in neonates to process and present antigen to CD8+ T cells remains to be addressed. We used human CD8+ T cell clones to compare the ability of neonatal and adult monocyte-derived dendritic cells to present or process and present antigen using the MHC class I pathway. Specifically, we assessed the ability of dendritic cells to present antigenic peptide, present an HLA-E-restricted antigen, process and present an MHC class I-restricted antigen through the classical MHC class I pathway, and cross present cell-associated antigen via MHC class I. We found no defect in neonatal dendritic cells to perform any of these processing and presentation functions and conclude that the MHC class I antigen processing and presentation pathway is functional in neonatal dendritic cells and hence may not account for the diminished control of pathogens.

Original languageEnglish (US)
Article numbere0000957
JournalPLoS One
Volume2
Issue number9
DOIs
StatePublished - 2007

Fingerprint

Histocompatibility Antigens Class I
Antigen Presentation
dendritic cells
Dendritic Cells
antigens
Antigens
Processing
T-cells
Pathogens
neonates
CD8 Antigens
T-Lymphocytes
T-lymphocytes
pathogens
Monocytes
Immunity
monocytes
Clone Cells
immunity
Defects

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Human Neonatal Dendritic Cells Are Competent in MHC Class I Antigen Processing and Presentation. / Gold, Marielle; Robinson, Tammie L.; Cook, Matthew S.; Byrd, Laura K.; Ehlinger, Heather D.; Lewinsohn, David; Lewinsohn, Deborah.

In: PLoS One, Vol. 2, No. 9, e0000957, 2007.

Research output: Contribution to journalArticle

Gold, Marielle ; Robinson, Tammie L. ; Cook, Matthew S. ; Byrd, Laura K. ; Ehlinger, Heather D. ; Lewinsohn, David ; Lewinsohn, Deborah. / Human Neonatal Dendritic Cells Are Competent in MHC Class I Antigen Processing and Presentation. In: PLoS One. 2007 ; Vol. 2, No. 9.
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