Human natural killer cells mediate adaptive immunity to viral antigens

Rana Nikzad, Laura S. Angelo, Kevin Aviles-Padilla, Duy T. Le, Vipul K. Singh, Lynn Bimler, Milica Vukmanovic-Stejic, Elena Vendrame, Thanmayi Ranganath, Laura Simpson, Nancy L. Haigwood, Catherine A. Blish, Arne N. Akbar, Silke Paust

    Research output: Contribution to journalArticle

    20 Scopus citations

    Abstract

    Adaptive immune responses are defined as antigen sensitization-dependent and antigen-specific responses leading to establishment of long-lived immunological memory. Although natural killer (NK) cells have traditionally been considered cells of the innate immune system, mounting evidence in mice and nonhuman primates warrants reconsideration of the existing paradigm that B and T cells are the sole mediators of adaptive immunity. However, it is currently unknown whether human NK cells can exhibit adaptive immune responses. We therefore tested whether human NK cells mediate adaptive immunity to virally encoded antigens using humanized mice and human volunteers. We found that human NK cells displayed vaccination-dependent, antigen-specific recall responses in vitro, when isolated from livers of humanized mice previously vaccinated with HIV-encoded envelope protein. Furthermore, we discovered that large numbers of cytotoxic NK cells with a tissue-resident phenotype were recruited to sites of varicella-zoster virus (VZV) skin test antigen challenge in VZV-experienced human volunteers. These NK-mediated recall responses in humans occurred decades after initial VZV exposure, demonstrating that NK memory in humans is long-lived. Our data demonstrate that human NK cells exhibit adaptive immune responses upon vaccination or infection. The existence of human memory NK cells may allow for the development of vaccination-based approaches capable of establishing potent NK-mediated memory functions contributing to host protection.

    Original languageEnglish (US)
    Article numbereaat8116
    JournalScience Immunology
    Volume4
    Issue number35
    DOIs
    StatePublished - Jan 1 2019

      Fingerprint

    ASJC Scopus subject areas

    • Immunology and Allergy
    • Immunology

    Cite this

    Nikzad, R., Angelo, L. S., Aviles-Padilla, K., Le, D. T., Singh, V. K., Bimler, L., Vukmanovic-Stejic, M., Vendrame, E., Ranganath, T., Simpson, L., Haigwood, N. L., Blish, C. A., Akbar, A. N., & Paust, S. (2019). Human natural killer cells mediate adaptive immunity to viral antigens. Science Immunology, 4(35), [eaat8116]. https://doi.org/10.1126/sciimmunol.aat8116