Human immunodeficiency virus type 1 neutralizing antibodies accelerate clearance of cell-free virions from blood plasma

Tatsuhiko Igarashi, Charles Brown, Ali Azadegan, Nancy Haigwood, Dimiter Dimitrov, Malcolm A. Martin, Riri Shibata

Research output: Contribution to journalArticle

131 Citations (Scopus)

Abstract

The concentration of human immunodeficiency virus type 1 (HIV-1) particles in blood plasma is very predictive of the subsequent disease course in an infected individual; its measurement has become one of the most important parameters for monitoring clinical status. Steady-state virus levels in plasma reflect a balance between the rates of virions entering and leaving the peripheral blood. We analyzed the rate of virus clearance in the general circulation in rhesus macaques receiving a continuous infusion of cell-free particles in the presence and absence of virus-specific antibodies. Here we show, by measuring virion RNA, particle-associated p24 Gag protein and virus infectivity, that the clearance of physical and infectious particles from a primary, dual-tropic virus isolate, HIV-1(DH12), is very rapid in naive animals, with half-lives ranging from 13 to 26 minutes. In the presence of high-titer HIV-1(DH12)-specific neutralizing antibodies, the half-life of virion RNA was considerably reduced (to 3.9-7.2 minutes), and infectious virus in the blood became undetectable. Although physical virus particles were eliminated extravascularly, the loss of virus infectivity in the blood reflected the combined effects of extravascular clearance and intravascular inactivation of HIV-1 infectivity due to antibody binding.

Original languageEnglish (US)
Pages (from-to)211-216
Number of pages6
JournalNature Medicine
Volume5
Issue number2
DOIs
StatePublished - Feb 1999
Externally publishedYes

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Neutralizing Antibodies
Viruses
Virion
HIV-1
Blood
Plasmas
RNA
gag Gene Products
Antibodies
Macaca mulatta
Half-Life
Tropics
Animals

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Human immunodeficiency virus type 1 neutralizing antibodies accelerate clearance of cell-free virions from blood plasma. / Igarashi, Tatsuhiko; Brown, Charles; Azadegan, Ali; Haigwood, Nancy; Dimitrov, Dimiter; Martin, Malcolm A.; Shibata, Riri.

In: Nature Medicine, Vol. 5, No. 2, 02.1999, p. 211-216.

Research output: Contribution to journalArticle

Igarashi, Tatsuhiko ; Brown, Charles ; Azadegan, Ali ; Haigwood, Nancy ; Dimitrov, Dimiter ; Martin, Malcolm A. ; Shibata, Riri. / Human immunodeficiency virus type 1 neutralizing antibodies accelerate clearance of cell-free virions from blood plasma. In: Nature Medicine. 1999 ; Vol. 5, No. 2. pp. 211-216.
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