Human DNA polymerase alpha gene expression is cell proliferation dependent and its primary structure is similar to both prokaryotic and eukaryotic replicative DNA polymerases.

Scott Wong, A. F. Wahl, P. M. Yuan, N. Arai, B. E. Pearson, K. Arai, D. Korn, M. W. Hunkapiller, T. S. Wang

Research output: Contribution to journalArticle

292 Citations (Scopus)

Abstract

We have isolated cDNA clones encoding the human DNA polymerase alpha catalytic polypeptide. Studies of the human DNA polymerase alpha steady-state mRNA levels in quiescent cells stimulated to proliferate, or normal cells compared to transformed cells, demonstrate that the polymerase alpha mRNA, like its enzymatic activity and de novo protein synthesis, positively correlates with cell proliferation and transformation. Analysis of the deduced 1462-amino-acid sequence reveals six regions of striking similarity to yeast DNA polymerase I and DNA polymerases of bacteriophages T4 and phi 29, herpes family viruses, vaccinia virus and adenovirus. Three of these conserved regions appear to comprise the functional active site required for deoxynucleotide interaction. Two putative DNA interacting domains are also identified.

Original languageEnglish (US)
Pages (from-to)37-47
Number of pages11
JournalEMBO Journal
Volume7
Issue number1
StatePublished - Jan 1988
Externally publishedYes

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DNA Polymerase I
Cell proliferation
DNA-Directed DNA Polymerase
Gene expression
Cell Proliferation
Viruses
Gene Expression
Bacillus Phages
Bacteriophage T4
Messenger RNA
Bacteriophages
Vaccinia virus
Adenoviridae
Yeast
Amino Acid Sequence
Catalytic Domain
Complementary DNA
Clone Cells
Yeasts
Amino Acids

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Human DNA polymerase alpha gene expression is cell proliferation dependent and its primary structure is similar to both prokaryotic and eukaryotic replicative DNA polymerases. / Wong, Scott; Wahl, A. F.; Yuan, P. M.; Arai, N.; Pearson, B. E.; Arai, K.; Korn, D.; Hunkapiller, M. W.; Wang, T. S.

In: EMBO Journal, Vol. 7, No. 1, 01.1988, p. 37-47.

Research output: Contribution to journalArticle

Wong, Scott ; Wahl, A. F. ; Yuan, P. M. ; Arai, N. ; Pearson, B. E. ; Arai, K. ; Korn, D. ; Hunkapiller, M. W. ; Wang, T. S. / Human DNA polymerase alpha gene expression is cell proliferation dependent and its primary structure is similar to both prokaryotic and eukaryotic replicative DNA polymerases. In: EMBO Journal. 1988 ; Vol. 7, No. 1. pp. 37-47.
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