Human cytomegalovirus inhibits transcription of the CC chemokine MCP-1 gene

Alec Hirsch, Thomas Shenk

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

In primary human diploid fibroblasts, infection with an unpurified stock of human cytomegalovirus induced accumulation of the CC chemokine MCP-1 in the cell culture medium. By 24 h postinfection, the level of MCP-1 returned to that in uninfected cultures. When cells were infected with UV-inactivated human cytomegalovirus, the induction of MCP-1 was still observed, but no reduction was seen by 24 h postinfection or later. This effect was the result of a decrease in the level of MCP-1 mRNA present within the infected cell. Infection with purified virus revealed that the induction of MCP-1 was due to an activity found in the medium of infected cells; purified virions did not induce the expression of MCP-1. However, infection with purified virions repressed the level of MCP-1 mRNA below that found in uninfected cells. Additionally, infection with human cytomegalovirus prevented the induction of MCP-1 expression by tumor necrosis factor alpha and interleukin-1β. The CC chemokine receptor encoded by the human cytomegalovirus US28 open reading frame (ORF) did not appear to play a role in this process, since a mutant virus in which the US28 ORF had been deleted downregulated MCP-1 in the same manner.

Original languageEnglish (US)
Pages (from-to)404-410
Number of pages7
JournalJournal of Virology
Volume73
Issue number1
StatePublished - 1999
Externally publishedYes

Fingerprint

Human herpesvirus 5
CC Chemokines
1-methylcyclopropene
Cytomegalovirus
transcription (genetics)
Genes
Infection
genes
Virion
Open Reading Frames
CCR Receptors
Virus Activation
Messenger RNA
virion
infection
Diploidy
Interleukin-1
open reading frames
Culture Media
Down-Regulation

ASJC Scopus subject areas

  • Immunology

Cite this

Human cytomegalovirus inhibits transcription of the CC chemokine MCP-1 gene. / Hirsch, Alec; Shenk, Thomas.

In: Journal of Virology, Vol. 73, No. 1, 1999, p. 404-410.

Research output: Contribution to journalArticle

@article{f94fcf6131e64cf78a9f1ab3031c62a0,
title = "Human cytomegalovirus inhibits transcription of the CC chemokine MCP-1 gene",
abstract = "In primary human diploid fibroblasts, infection with an unpurified stock of human cytomegalovirus induced accumulation of the CC chemokine MCP-1 in the cell culture medium. By 24 h postinfection, the level of MCP-1 returned to that in uninfected cultures. When cells were infected with UV-inactivated human cytomegalovirus, the induction of MCP-1 was still observed, but no reduction was seen by 24 h postinfection or later. This effect was the result of a decrease in the level of MCP-1 mRNA present within the infected cell. Infection with purified virus revealed that the induction of MCP-1 was due to an activity found in the medium of infected cells; purified virions did not induce the expression of MCP-1. However, infection with purified virions repressed the level of MCP-1 mRNA below that found in uninfected cells. Additionally, infection with human cytomegalovirus prevented the induction of MCP-1 expression by tumor necrosis factor alpha and interleukin-1β. The CC chemokine receptor encoded by the human cytomegalovirus US28 open reading frame (ORF) did not appear to play a role in this process, since a mutant virus in which the US28 ORF had been deleted downregulated MCP-1 in the same manner.",
author = "Alec Hirsch and Thomas Shenk",
year = "1999",
language = "English (US)",
volume = "73",
pages = "404--410",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - Human cytomegalovirus inhibits transcription of the CC chemokine MCP-1 gene

AU - Hirsch, Alec

AU - Shenk, Thomas

PY - 1999

Y1 - 1999

N2 - In primary human diploid fibroblasts, infection with an unpurified stock of human cytomegalovirus induced accumulation of the CC chemokine MCP-1 in the cell culture medium. By 24 h postinfection, the level of MCP-1 returned to that in uninfected cultures. When cells were infected with UV-inactivated human cytomegalovirus, the induction of MCP-1 was still observed, but no reduction was seen by 24 h postinfection or later. This effect was the result of a decrease in the level of MCP-1 mRNA present within the infected cell. Infection with purified virus revealed that the induction of MCP-1 was due to an activity found in the medium of infected cells; purified virions did not induce the expression of MCP-1. However, infection with purified virions repressed the level of MCP-1 mRNA below that found in uninfected cells. Additionally, infection with human cytomegalovirus prevented the induction of MCP-1 expression by tumor necrosis factor alpha and interleukin-1β. The CC chemokine receptor encoded by the human cytomegalovirus US28 open reading frame (ORF) did not appear to play a role in this process, since a mutant virus in which the US28 ORF had been deleted downregulated MCP-1 in the same manner.

AB - In primary human diploid fibroblasts, infection with an unpurified stock of human cytomegalovirus induced accumulation of the CC chemokine MCP-1 in the cell culture medium. By 24 h postinfection, the level of MCP-1 returned to that in uninfected cultures. When cells were infected with UV-inactivated human cytomegalovirus, the induction of MCP-1 was still observed, but no reduction was seen by 24 h postinfection or later. This effect was the result of a decrease in the level of MCP-1 mRNA present within the infected cell. Infection with purified virus revealed that the induction of MCP-1 was due to an activity found in the medium of infected cells; purified virions did not induce the expression of MCP-1. However, infection with purified virions repressed the level of MCP-1 mRNA below that found in uninfected cells. Additionally, infection with human cytomegalovirus prevented the induction of MCP-1 expression by tumor necrosis factor alpha and interleukin-1β. The CC chemokine receptor encoded by the human cytomegalovirus US28 open reading frame (ORF) did not appear to play a role in this process, since a mutant virus in which the US28 ORF had been deleted downregulated MCP-1 in the same manner.

UR - http://www.scopus.com/inward/record.url?scp=0032889030&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032889030&partnerID=8YFLogxK

M3 - Article

VL - 73

SP - 404

EP - 410

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 1

ER -