Abstract
Base editing installs a precise nucleotide change in specific gene loci without causing a double-strand break. Its efficiency in human embryos is generally low, limiting its utility in functional genetic studies. Here, we report that injecting base editors into human cleaving two-cell and four-cell embryos results in much higher (up to 13-fold) homozygotic nucleotide substitution efficiency as opposed to MII oocytes or zygotes. Furthermore, as a proof-of-principle study, a point mutation can be efficiently corrected by our method. Our study indicates that human cleaving embryos provide an efficient base editing window for robust gene disruption and correction.
Original language | English (US) |
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Article number | 101 |
Journal | Genome biology |
Volume | 20 |
Issue number | 1 |
DOIs | |
State | Published - May 22 2019 |
Keywords
- Base editing
- Homozygotic nucleotide substitution
- Human cleaving embryos
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Genetics
- Cell Biology