@article{c4c2ae0c17f44000906277a0018ce6fe,
title = "Human cleaving embryos enable robust homozygotic nucleotide substitutions by base editors",
abstract = "Base editing installs a precise nucleotide change in specific gene loci without causing a double-strand break. Its efficiency in human embryos is generally low, limiting its utility in functional genetic studies. Here, we report that injecting base editors into human cleaving two-cell and four-cell embryos results in much higher (up to 13-fold) homozygotic nucleotide substitution efficiency as opposed to MII oocytes or zygotes. Furthermore, as a proof-of-principle study, a point mutation can be efficiently corrected by our method. Our study indicates that human cleaving embryos provide an efficient base editing window for robust gene disruption and correction.",
keywords = "Base editing, Homozygotic nucleotide substitution, Human cleaving embryos",
author = "Meiling Zhang and Changyang Zhou and Yu Wei and Chunlong Xu and Hong Pan and Wenqin Ying and Yidi Sun and Yun Sun and Qingquan Xiao and Ning Yao and Wanxia Zhong and Yun Li and Keliang Wu and Gao Yuan and Shoukhrat Mitalipov and Chen, {Zi Jiang} and Hui Yang",
note = "Funding Information: This work was supported by the R&D Program of China (2018YFC2000100 and 2017YFC1001302 to HY; 2016YFC1000604 to ZJC), CAS Strategic Priority Research Program (XDB32060000), National Natural Science Foundation of China (31871502, 31522037), Shanghai Municipal Science and Technology Major Project (2018SHZDZX05), Shanghai City Committee of Science and Technology Project (18411953700, 18JC1410100), and Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetic (17DZ2271100). Publisher Copyright: {\textcopyright} 2019 The Author(s).",
year = "2019",
month = may,
day = "22",
doi = "10.1186/s13059-019-1703-6",
language = "English (US)",
volume = "20",
journal = "Genome Biology",
issn = "1474-7596",
publisher = "BioMed Central",
number = "1",
}