Histone demethylase KDM4A and KDM4B expression in granulosa cells from women undergoing in vitro fertilization

Adam Krieg, Sarah R. Mullinax, Frances Grimstad, Kaitlin Marquis, Elizabeth Constance, Yan Hong, Sacha A. Krieg, Katherine F. Roby

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Purpose: To assess expression of the histone demethylases KDM4A and KDM4B in granulosa collected from women undergoing oocyte retrieval and to determine if expression was related to pregnancy outcome. Methods: Cumulus and mural granulosa cells were obtained from women undergoing oocyte retrieval. KDM4A and KDM4B mRNA expression was determined by qRT-PCR. KDM4A and KDM4B proteins were immunohistochemically localized in ovarian tissue sections obtained from archival specimens. Results: KDM4A and KDM4B protein was localized to oocytes, granulosa cells, and theca and luteal cells in ovaries from reproductive-aged women. KDM4A and KDM4B mRNA expression was overall higher in cumulus compared to mural granulosa. When comparing granulosa demethylase gene expression, KDM4A and KDM4B mRNA expression was higher in both cumulus and mural granulosa from not pregnant patients compared to patients in the pregnant-live birth group. Conclusions: Histone demethylases KDM4A and KDM4B mRNA are differentially expressed in cumulus and mural granulosa. Expression of both KDM4A and KDM4B mRNA was lower in cumulus granulosa and mural granulosa from pregnant compared to not pregnant patients. These findings suggest that altered expression of histone demethylases may impact epigenetic changes in granulosa cells associated with pregnancy.

Original languageEnglish (US)
Pages (from-to)993-1003
Number of pages11
JournalJournal of Assisted Reproduction and Genetics
Volume35
Issue number6
DOIs
StatePublished - Jun 1 2018

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Histone Demethylases
Granulosa Cells
Fertilization in Vitro
Messenger RNA
Oocyte Retrieval
Cumulus Cells
Luteal Cells
Live Birth
Pregnancy Outcome
Epigenomics
Oocytes
Ovary
Proteins
Gene Expression
Pregnancy
Polymerase Chain Reaction

Keywords

  • Granulosa
  • Histone demethylase
  • IVF
  • KDM4
  • Ovary
  • Pregnancy

ASJC Scopus subject areas

  • Reproductive Medicine
  • Genetics
  • Obstetrics and Gynecology
  • Developmental Biology
  • Genetics(clinical)

Cite this

Histone demethylase KDM4A and KDM4B expression in granulosa cells from women undergoing in vitro fertilization. / Krieg, Adam; Mullinax, Sarah R.; Grimstad, Frances; Marquis, Kaitlin; Constance, Elizabeth; Hong, Yan; Krieg, Sacha A.; Roby, Katherine F.

In: Journal of Assisted Reproduction and Genetics, Vol. 35, No. 6, 01.06.2018, p. 993-1003.

Research output: Contribution to journalArticle

Krieg, Adam ; Mullinax, Sarah R. ; Grimstad, Frances ; Marquis, Kaitlin ; Constance, Elizabeth ; Hong, Yan ; Krieg, Sacha A. ; Roby, Katherine F. / Histone demethylase KDM4A and KDM4B expression in granulosa cells from women undergoing in vitro fertilization. In: Journal of Assisted Reproduction and Genetics. 2018 ; Vol. 35, No. 6. pp. 993-1003.
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abstract = "Purpose: To assess expression of the histone demethylases KDM4A and KDM4B in granulosa collected from women undergoing oocyte retrieval and to determine if expression was related to pregnancy outcome. Methods: Cumulus and mural granulosa cells were obtained from women undergoing oocyte retrieval. KDM4A and KDM4B mRNA expression was determined by qRT-PCR. KDM4A and KDM4B proteins were immunohistochemically localized in ovarian tissue sections obtained from archival specimens. Results: KDM4A and KDM4B protein was localized to oocytes, granulosa cells, and theca and luteal cells in ovaries from reproductive-aged women. KDM4A and KDM4B mRNA expression was overall higher in cumulus compared to mural granulosa. When comparing granulosa demethylase gene expression, KDM4A and KDM4B mRNA expression was higher in both cumulus and mural granulosa from not pregnant patients compared to patients in the pregnant-live birth group. Conclusions: Histone demethylases KDM4A and KDM4B mRNA are differentially expressed in cumulus and mural granulosa. Expression of both KDM4A and KDM4B mRNA was lower in cumulus granulosa and mural granulosa from pregnant compared to not pregnant patients. These findings suggest that altered expression of histone demethylases may impact epigenetic changes in granulosa cells associated with pregnancy.",
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AU - Marquis, Kaitlin

AU - Constance, Elizabeth

AU - Hong, Yan

AU - Krieg, Sacha A.

AU - Roby, Katherine F.

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AB - Purpose: To assess expression of the histone demethylases KDM4A and KDM4B in granulosa collected from women undergoing oocyte retrieval and to determine if expression was related to pregnancy outcome. Methods: Cumulus and mural granulosa cells were obtained from women undergoing oocyte retrieval. KDM4A and KDM4B mRNA expression was determined by qRT-PCR. KDM4A and KDM4B proteins were immunohistochemically localized in ovarian tissue sections obtained from archival specimens. Results: KDM4A and KDM4B protein was localized to oocytes, granulosa cells, and theca and luteal cells in ovaries from reproductive-aged women. KDM4A and KDM4B mRNA expression was overall higher in cumulus compared to mural granulosa. When comparing granulosa demethylase gene expression, KDM4A and KDM4B mRNA expression was higher in both cumulus and mural granulosa from not pregnant patients compared to patients in the pregnant-live birth group. Conclusions: Histone demethylases KDM4A and KDM4B mRNA are differentially expressed in cumulus and mural granulosa. Expression of both KDM4A and KDM4B mRNA was lower in cumulus granulosa and mural granulosa from pregnant compared to not pregnant patients. These findings suggest that altered expression of histone demethylases may impact epigenetic changes in granulosa cells associated with pregnancy.

KW - Granulosa

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KW - KDM4

KW - Ovary

KW - Pregnancy

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