High molecular weight chitosan derivative polymeric micelles encapsulating superparamagnetic iron oxide for tumor-targeted magnetic resonance imaging

Yunbin Xiao, Zuan Tao Lin, Yanmei Chen, He Wang, Ya Li Deng, Dai-Trang (Elizabeth) Le, Jianguo Bin, Meiyu Li, Yulin Liao, Yili Liu, Gangbiao Jiang, Jianping Bin

    Research output: Contribution to journalArticle

    22 Citations (Scopus)

    Abstract

    Magnetic resonance imaging (MRI) contrast agents based on chitosan derivatives have great potential for diagnosing diseases. However, stable tumor-targeted MRI contrast agents using micelles prepared from high molecular weight chitosan derivatives are seldom reported. In this study, we developed a novel tumor-targeted MRI vehicle via superparamagnetic iron oxide nanoparticles (SPIONs) encapsulated in self-aggregating polymeric folate-conjugated N-palmitoyl chitosan (FAPLCS) micelles. The tumor-targeting ability of FAPLCS/SPIONs was demonstrated in vitro and in vivo. The results of dynamic light scattering experiments showed that the micelles had a relatively narrow size distribution (136.60±3.90 nm) and excellent stability. FAPLCS/SPIONs showed low cytotoxicity and excellent biocompatibility in cellular toxicity tests. Both in vitro and in vivo studies demonstrated that FAPLCS/SPIONs bound specifically to folate receptor-positive HeLa cells, and that FAPLCS/SPIONs accumulated predominantly in established HeLa-derived tumors in mice. The signal intensities of T2-weighted images in established HeLa-derived tumors were reduced dramatically after intravenous micelle administration. Our study indicates that FAPLCS/SPION micelles can potentially serve as safe and effective MRI contrast agents for detecting tumors that overexpress folate receptors.

    Original languageEnglish (US)
    Pages (from-to)1155-1172
    Number of pages18
    JournalInternational Journal of Nanomedicine
    Volume10
    DOIs
    StatePublished - Feb 5 2015

    Fingerprint

    Chitosan
    Micelles
    Magnetic resonance
    Iron oxides
    Folic Acid
    Tumors
    Molecular Weight
    Molecular weight
    Magnetic Resonance Imaging
    Nanoparticles
    Derivatives
    Imaging techniques
    Magnetic resonance imaging
    Neoplasms
    Contrast Media
    Toxicity Tests
    ferric oxide
    Dynamic light scattering
    Cytotoxicity
    Biocompatibility

    Keywords

    • Folate receptors
    • Magnetic resonance imaging
    • N-palmitoyl chitosan
    • Polymeric micelles
    • Superparamagnetic iron oxide
    • Tumor-targeted MRI

    ASJC Scopus subject areas

    • Biophysics
    • Bioengineering
    • Biomaterials
    • Drug Discovery
    • Organic Chemistry

    Cite this

    High molecular weight chitosan derivative polymeric micelles encapsulating superparamagnetic iron oxide for tumor-targeted magnetic resonance imaging. / Xiao, Yunbin; Lin, Zuan Tao; Chen, Yanmei; Wang, He; Deng, Ya Li; Le, Dai-Trang (Elizabeth); Bin, Jianguo; Li, Meiyu; Liao, Yulin; Liu, Yili; Jiang, Gangbiao; Bin, Jianping.

    In: International Journal of Nanomedicine, Vol. 10, 05.02.2015, p. 1155-1172.

    Research output: Contribution to journalArticle

    Xiao, Yunbin ; Lin, Zuan Tao ; Chen, Yanmei ; Wang, He ; Deng, Ya Li ; Le, Dai-Trang (Elizabeth) ; Bin, Jianguo ; Li, Meiyu ; Liao, Yulin ; Liu, Yili ; Jiang, Gangbiao ; Bin, Jianping. / High molecular weight chitosan derivative polymeric micelles encapsulating superparamagnetic iron oxide for tumor-targeted magnetic resonance imaging. In: International Journal of Nanomedicine. 2015 ; Vol. 10. pp. 1155-1172.
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