High molecular weight chitosan derivative polymeric micelles encapsulating superparamagnetic iron oxide for tumor-targeted magnetic resonance imaging

Yunbin Xiao, Zuan Tao Lin, Yanmei Chen, He Wang, Ya Li Deng, D. Elizabeth Le, Jianguo Bin, Meiyu Li, Yulin Liao, Yili Liu, Gangbiao Jiang, Jianping Bin

    Research output: Contribution to journalArticle

    24 Scopus citations

    Abstract

    Magnetic resonance imaging (MRI) contrast agents based on chitosan derivatives have great potential for diagnosing diseases. However, stable tumor-targeted MRI contrast agents using micelles prepared from high molecular weight chitosan derivatives are seldom reported. In this study, we developed a novel tumor-targeted MRI vehicle via superparamagnetic iron oxide nanoparticles (SPIONs) encapsulated in self-aggregating polymeric folate-conjugated N-palmitoyl chitosan (FAPLCS) micelles. The tumor-targeting ability of FAPLCS/SPIONs was demonstrated in vitro and in vivo. The results of dynamic light scattering experiments showed that the micelles had a relatively narrow size distribution (136.60±3.90 nm) and excellent stability. FAPLCS/SPIONs showed low cytotoxicity and excellent biocompatibility in cellular toxicity tests. Both in vitro and in vivo studies demonstrated that FAPLCS/SPIONs bound specifically to folate receptor-positive HeLa cells, and that FAPLCS/SPIONs accumulated predominantly in established HeLa-derived tumors in mice. The signal intensities of T2-weighted images in established HeLa-derived tumors were reduced dramatically after intravenous micelle administration. Our study indicates that FAPLCS/SPION micelles can potentially serve as safe and effective MRI contrast agents for detecting tumors that overexpress folate receptors.

    Original languageEnglish (US)
    Pages (from-to)1155-1172
    Number of pages18
    JournalInternational journal of nanomedicine
    Volume10
    DOIs
    StatePublished - Feb 5 2015

    Keywords

    • Folate receptors
    • Magnetic resonance imaging
    • N-palmitoyl chitosan
    • Polymeric micelles
    • Superparamagnetic iron oxide
    • Tumor-targeted MRI

    ASJC Scopus subject areas

    • Biophysics
    • Bioengineering
    • Biomaterials
    • Pharmaceutical Science
    • Drug Discovery
    • Organic Chemistry

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