@article{49bcd0e2b3f84e878c1777909930f4b1,
title = "High level production, characterization and construct optimization of the ionotropic glutamate receptor ligand binding core",
abstract = "Multimilligram quantities of active and pure GluR2-S1S2, the recombinant ligand binding core of the AMPA-sensitive GluR2 receptor, were produced by preparative folding of the solubilized inclusion bodies expressed in 1 l of Escherichia coli cell culture. The biochemical properties and biological activities of folded protein were characterized and the protein construct was optimized for three-dimensional structural studies. (C) 2000 Elsevier Science Ltd.",
keywords = "Escherichia coli, Glutamate, Ligand binding, Preparative folding, Protein engineering, Protein production, Receptor, X-ray crystallography",
author = "Chen, {Guo Qiang} and Eric Gouaux",
note = "Funding Information: Drs Hartley and Heinemann are acknowledged for providing the rat GluR2 clone. We would like to thank Mr Sun and Mr Jin for cloning and ligand binding experiments of the HS1S2I construct, Ms Gawinowicz for N-terminal sequence and MALDI-MS analyses and Dr Ming-Ming Zhou for the NMR spectra. We are also grateful to Drs Karlin, Javitch, Hendrickson, Hirsh, Palmer, Pyle, and Thanos for their helpful assistance and for equipment use. Support for this research was provided by a NSF Young Investigator Award, an Alfred P. Sloan Research Fellowship, the Klingenstein Foundation and the National Alliance for Research on Schizophrenia and Depression.",
year = "2000",
month = nov,
day = "24",
doi = "10.1016/S0040-4020(00)00825-5",
language = "English (US)",
volume = "56",
pages = "9409--9419",
journal = "Tetrahedron",
issn = "0040-4020",
publisher = "Elsevier Limited",
number = "48",
}