High level production, characterization and construct optimization of the ionotropic glutamate receptor ligand binding core

Guo Qiang Chen, Eric Gouaux

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Multimilligram quantities of active and pure GluR2-S1S2, the recombinant ligand binding core of the AMPA-sensitive GluR2 receptor, were produced by preparative folding of the solubilized inclusion bodies expressed in 1 l of Escherichia coli cell culture. The biochemical properties and biological activities of folded protein were characterized and the protein construct was optimized for three-dimensional structural studies. (C) 2000 Elsevier Science Ltd.

Original languageEnglish (US)
Pages (from-to)9409-9419
Number of pages11
JournalTetrahedron
Volume56
Issue number48
DOIs
StatePublished - Nov 24 2000

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Keywords

  • Escherichia coli
  • Glutamate
  • Ligand binding
  • Preparative folding
  • Protein engineering
  • Protein production
  • Receptor
  • X-ray crystallography

ASJC Scopus subject areas

  • Biochemistry
  • Drug Discovery
  • Organic Chemistry

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