High frequency of virus-specific B lymphocytes in germinal centers of simian-human immunodeficiency virus-infected rhesus monkeys

David H. Margolin, Erika F. Helmuth Saunders, Benjamin Bronfin, Nicole De Rosa, Michael Axthelm, Xavier Alvarez, Norman L. Letvin

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The etiology of the lymphadenopathy and follicular hyperplasia associated with human immunodeficiency virus type 1 (HIV-1) infection has remained unclear. To determine whether the B-lymphocyte expansions characteristic of this syndrome represent polyclonal and virus-specific processes, the antigen specificity of B cells in lymphoid tissues of monkeys infected with simian-human immunodeficiency virus (SHIV) chimeras was assessed using an inverse immunohistochemical assay with biotinylated HIV-1 envelope gp120 (Env) as an antigen probe. Env-binding B cells were found aggregated in lymph node and splenic germinal centers (GCs). Most Env-binding GCs also contained an unstained population of B cells, suggesting the GCs were formed by a polyclonal (oligoclonal) process. By day 42 following infection, Env-binding B cells were present in 19% of all lymph node GCs. Env-binding cells were present in 25% of GCs even during chronic infection. This extraordinarily high frequency of Env-specific B lymphocytes suggests that the expansion of virus-specific B cells may largely account for the follicular hyperplasia in AIDS virus-infected individuals.

Original languageEnglish (US)
Pages (from-to)3965-3973
Number of pages9
JournalJournal of Virology
Volume76
Issue number8
DOIs
StatePublished - 2002
Externally publishedYes

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Simian Immunodeficiency Virus
Germinal Center
Human immunodeficiency virus
Macaca mulatta
B-lymphocytes
B-Lymphocytes
HIV
Viruses
viruses
Human immunodeficiency virus 1
hyperplasia
Hyperplasia
lymph nodes
HIV-1
Lymph Nodes
infection
env Gene Products
antigens
lymphatic diseases
chimerism

ASJC Scopus subject areas

  • Immunology

Cite this

High frequency of virus-specific B lymphocytes in germinal centers of simian-human immunodeficiency virus-infected rhesus monkeys. / Margolin, David H.; Helmuth Saunders, Erika F.; Bronfin, Benjamin; De Rosa, Nicole; Axthelm, Michael; Alvarez, Xavier; Letvin, Norman L.

In: Journal of Virology, Vol. 76, No. 8, 2002, p. 3965-3973.

Research output: Contribution to journalArticle

Margolin, David H. ; Helmuth Saunders, Erika F. ; Bronfin, Benjamin ; De Rosa, Nicole ; Axthelm, Michael ; Alvarez, Xavier ; Letvin, Norman L. / High frequency of virus-specific B lymphocytes in germinal centers of simian-human immunodeficiency virus-infected rhesus monkeys. In: Journal of Virology. 2002 ; Vol. 76, No. 8. pp. 3965-3973.
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