Hexagonal organization of Moloney murine leukemia virus capsid proteins

Keith Mayo, Jason McDermott, Eric Barklis

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

To help elucidate the mechanisms by which retrovirus structural proteins associate to form virus particles, we have examined membrane-bound assemblies of Moloney murine leukemia virus (M-MuLV) capsid (CA) proteins. Electron microscopy and image reconstruction techniques showed that CA dimers appear to function as organizational subunits of the cage-like, membrane-bound protein arrays. However, new three-dimensional (3D) data also were consistent with hexagonal (p6) assembly models. The p6 3D reconstructions of membrane-bound M-MuLV CA proteins gave unit cells of a = b = 80.3 Å, c = 110 Å, γ = 120°, in which six dimer units formed a cage lattice. Neighbor cage hole-to-hole distances were 45 Å, while distances between hexagonal cage holes corresponded to unit cell lengths (80.3 Å). The hexagonal model predicts two types of cage holes (trimer and hexamer holes), uses symmetric head-to-head dimer building blocks, and permits the introduction of lattice curvature by conversion of hexamer to pentamer units. The M-MuLV CA lattice is similar to those formed in helical tubes by HIV CA in that hexamer units surround cage holes of 25-30 Å, but differs in that M-MuLV hexamer units appear to be CA dimers, whereas HIV CA units appear to be monomers. These results suggest that while general assembly principles apply to different retroviruses, clear assembly distinctions exist between these virus types.

Original languageEnglish (US)
Pages (from-to)30-38
Number of pages9
JournalVirology
Volume298
Issue number1
DOIs
StatePublished - 2002

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Moloney murine leukemia virus
Capsid
Capsid Proteins
Retroviridae Proteins
HIV
Protein Array Analysis
Computer-Assisted Image Processing
Membranes
Retroviridae
Virion
Electron Microscopy
Membrane Proteins
Viruses

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Hexagonal organization of Moloney murine leukemia virus capsid proteins. / Mayo, Keith; McDermott, Jason; Barklis, Eric.

In: Virology, Vol. 298, No. 1, 2002, p. 30-38.

Research output: Contribution to journalArticle

Mayo, Keith ; McDermott, Jason ; Barklis, Eric. / Hexagonal organization of Moloney murine leukemia virus capsid proteins. In: Virology. 2002 ; Vol. 298, No. 1. pp. 30-38.
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