Herpes simplex viruses lacking glycoprotein D are unable to inhibit virus penetration: Quantitative evidence for virus-specific cell surface receptors

David Johnson, M. W. Ligas

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122 Citations (Scopus)

Abstract

Herpes simplex virus (HSV) glycoprotein D (gD) plays an essential role in the entry of virus into cells. HSV mutants unable to express gD were constructed. The mutants can be propagated on VD60 cells, which supply the viruses with gD; however, virus particles lacking gD were produced in mutant-infected Vero cells. Virus particles with or without gD adsorbed to a large number (>4 x 104) of sites on the cell surface; however, virions lacking gD did not enter cells. Cells pretreated with UV-inactivated virions containing gD (~5 x 103 particles per cell) were resistant to infection with HSV type 1 (HSV-1) and HSV-2. In contrast, cells pretreated with UV-inactivated virions lacking gD could be infected with HSV-1 and HSV-2. If infectious HSV-1 was added prior to UV-inactivated virus particles containing gD, the infectious virus entered cells and replicated. Therefore, virus particles containing gD appear to block specific cell surface receptors which are very limited in number. Particles lacking gD are presumably unable to interact with these receptors, suggesting that gD is an essential receptor-binding polypeptide.

Original languageEnglish (US)
Pages (from-to)4605-4612
Number of pages8
JournalJournal of Virology
Volume62
Issue number12
StatePublished - 1988
Externally publishedYes

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herpes simplex
Cell Surface Receptors
Simplexvirus
glycoproteins
Glycoproteins
Viruses
viruses
receptors
virion
Virion
cells
Human herpesvirus 1
Human Herpesvirus 1
Human herpesvirus 2
Human Herpesvirus 2
mutants
Virus Internalization
Vero Cells
polypeptides

ASJC Scopus subject areas

  • Immunology

Cite this

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N2 - Herpes simplex virus (HSV) glycoprotein D (gD) plays an essential role in the entry of virus into cells. HSV mutants unable to express gD were constructed. The mutants can be propagated on VD60 cells, which supply the viruses with gD; however, virus particles lacking gD were produced in mutant-infected Vero cells. Virus particles with or without gD adsorbed to a large number (>4 x 104) of sites on the cell surface; however, virions lacking gD did not enter cells. Cells pretreated with UV-inactivated virions containing gD (~5 x 103 particles per cell) were resistant to infection with HSV type 1 (HSV-1) and HSV-2. In contrast, cells pretreated with UV-inactivated virions lacking gD could be infected with HSV-1 and HSV-2. If infectious HSV-1 was added prior to UV-inactivated virus particles containing gD, the infectious virus entered cells and replicated. Therefore, virus particles containing gD appear to block specific cell surface receptors which are very limited in number. Particles lacking gD are presumably unable to interact with these receptors, suggesting that gD is an essential receptor-binding polypeptide.

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