Heme oxygenase expression in human placenta and placental bed: Reduced expression of placenta endothelial HO-2 in preeclampsia and fetal growth restriction

Andrew Barber, Stephen Courtenay Robson, Leslie Myatt, Judith Nicola Bulmer, Fiona Lyall

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

In this study we tested the hypothesis that expression of heme oxygenases HO-1 and HO-2, which are responsible for the production of carbon monoxide, are reduced in the placenta and placental bed of pregnancies complicated by preeclampsia (PE) and fetal growth restriction (FGR) compared with control third-trimester pregnancies. Placental protein expression was determined by Western blotting (n=10 in each group) and immunohistochemistry (controls n=18, PE n=19, FGR n=10). Extravillous trophoblast expression was determined by immunohistochemistry of placental bed biopsy samples (controls n=17, PE n=19, FGR n=10). Western blot analysis of placental homogenates showed no overall differences in HO-2 among groups. However, immunohistochemical analysis showed a reduction in HO-2 expression in endothelial cells in both abnormal groups (PE P

Original languageEnglish (US)
Pages (from-to)1158-1168
Number of pages11
JournalFASEB Journal
Volume15
Issue number7
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

pre-eclampsia
Heme Oxygenase (Decyclizing)
fetal development
heme
oxygenases
Fetal Development
Pre-Eclampsia
placenta
Placenta
Pregnancy Proteins
heme oxygenase (biliverdin-producing)
immunohistochemistry
Western blotting
Biopsy
Western Blotting
Immunohistochemistry
Endothelial cells
pregnancy
Carbon Monoxide
Heme Oxygenase-1

Keywords

  • Carbon monoxide
  • HO-1
  • HO-2
  • Pregnancy
  • Trophoblast

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Heme oxygenase expression in human placenta and placental bed : Reduced expression of placenta endothelial HO-2 in preeclampsia and fetal growth restriction. / Barber, Andrew; Robson, Stephen Courtenay; Myatt, Leslie; Bulmer, Judith Nicola; Lyall, Fiona.

In: FASEB Journal, Vol. 15, No. 7, 2001, p. 1158-1168.

Research output: Contribution to journalArticle

Barber, Andrew ; Robson, Stephen Courtenay ; Myatt, Leslie ; Bulmer, Judith Nicola ; Lyall, Fiona. / Heme oxygenase expression in human placenta and placental bed : Reduced expression of placenta endothelial HO-2 in preeclampsia and fetal growth restriction. In: FASEB Journal. 2001 ; Vol. 15, No. 7. pp. 1158-1168.
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