HCMV Infection of Humanized Mice after Transplantation of G-CSF-Mobilized Peripheral Blood Stem Cells from HCMV-Seropositive Donors

Morgan Hakki, Devorah Goldman, Daniel Streblow, Kimberly L. Hamlin, Craig N. Krekylwich, William Fleming, Jay Nelson

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Human cytomegalovirus (HCMV) infection, including primary infection resulting from transmission from a seropositive donor to a seronegative recipient (D+/R-), remains a significant problem in the setting of peripheral blood stem cell transplantation (PBSCT). The lack of a suitable animal model for studying HCMV transmission after PBSCT is a major barrier to understanding this process and, consequently, developing novel interventions to prevent HCMV infection. Our previous work demonstrated that human CD34+ progenitor cell-engrafted NOD-scid IL2Rγcnull (NSG) mice support latent HCMV infection after direct inoculation and reactivation after treatment with granulocyte colony-stimulating factor. To more accurately recapitulate HCMV infection in the D+/R- PBSCT setting, granulocyte colony-stimulating factor-mobilized peripheral blood stem cells from seropositive donors were used to engraft NSG mice. All recipient mice demonstrated evidence of HCMV infection in liver, spleen, and bone marrow. These findings validate the NSG mouse model for studying HCMV transmission during PBSCT.

Original languageEnglish (US)
Pages (from-to)132-135
Number of pages4
JournalBiology of Blood and Marrow Transplantation
Volume20
Issue number1
DOIs
StatePublished - Jan 2014

Fingerprint

Cytomegalovirus Infections
Granulocyte Colony-Stimulating Factor
Cytomegalovirus
Transplantation
Peripheral Blood Stem Cell Transplantation
Infectious Disease Transmission
Peripheral Blood Stem Cells
Stem Cells
Spleen
Animal Models
Bone Marrow
Liver

Keywords

  • Cytomegalovirus
  • Latency
  • NSG mouse model
  • Stem cell transplantation
  • Transmission

ASJC Scopus subject areas

  • Transplantation
  • Hematology

Cite this

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abstract = "Human cytomegalovirus (HCMV) infection, including primary infection resulting from transmission from a seropositive donor to a seronegative recipient (D+/R-), remains a significant problem in the setting of peripheral blood stem cell transplantation (PBSCT). The lack of a suitable animal model for studying HCMV transmission after PBSCT is a major barrier to understanding this process and, consequently, developing novel interventions to prevent HCMV infection. Our previous work demonstrated that human CD34+ progenitor cell-engrafted NOD-scid IL2Rγcnull (NSG) mice support latent HCMV infection after direct inoculation and reactivation after treatment with granulocyte colony-stimulating factor. To more accurately recapitulate HCMV infection in the D+/R- PBSCT setting, granulocyte colony-stimulating factor-mobilized peripheral blood stem cells from seropositive donors were used to engraft NSG mice. All recipient mice demonstrated evidence of HCMV infection in liver, spleen, and bone marrow. These findings validate the NSG mouse model for studying HCMV transmission during PBSCT.",
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AU - Fleming, William

AU - Nelson, Jay

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