Hair-bundle proteomes of avian and mammalian inner-ear utricles

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Abstract

Examination of multiple proteomics datasets within or between species increases the reliability of protein identification. We report here proteomes of inner-ear hair bundles from three species (chick, mouse, and rat), which were collected on LTQ or LTQ Velos ion-trap mass spectrometers; the constituent proteins were quantified using MS2 intensities, which are the summed intensities of all peptide fragmentation spectra matched to a protein. The data are available via ProteomeXchange with identifiers PXD002410 (chick LTQ), PXD002414 (chick Velos), PXD002415 (mouse Velos), and PXD002416 (rat LTQ). The two chick bundle datasets compared favourably to a third, already-described chick bundle dataset, which was quantified using MS1 peak intensities, the summed intensities of peptides identified by high-resolution mass spectrometry (PXD000104; updated analysis in PXD002445). The mouse bundle dataset described here was comparable to a different mouse bundle dataset quantified using MS1 intensities (PXD002167). These six datasets will be useful for identifying the core proteome of vestibular hair bundles.

Original languageEnglish (US)
Article number150074
JournalScientific data
Volume2
DOIs
StatePublished - Dec 8 2015

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Bundle
Proteins
Mouse
Peptides
Rats
Protein
Mass spectrometers
Proteomics
fragmentation
Mass Spectrometry
Mass spectrometry
Fragmentation
Trap
Spectrometer
Chick
High Resolution
examination
Ions
Rat

ASJC Scopus subject areas

  • Education
  • Library and Information Sciences
  • Computer Science Applications
  • Information Systems
  • Statistics, Probability and Uncertainty
  • Statistics and Probability

Cite this

Hair-bundle proteomes of avian and mammalian inner-ear utricles. / Wilmarth, Phillip; Krey, Jocelyn F.; Shin, Jung Bum; Choi, Dongseok; David, Larry; Barr-Gillespie, Peter.

In: Scientific data, Vol. 2, 150074, 08.12.2015.

Research output: Contribution to journalArticle

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AU - Barr-Gillespie, Peter

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