Growth hormone regulates the abundance of insulin-like growth factor I RNA in adult rat liver

C. T. Roberts, A. L. Brown, D. E. Graham, S. Seelig, S. Berry, K. H. Gabbay, M. M. Rechler

Research output: Contribution to journalArticlepeer-review

153 Scopus citations

Abstract

Insulin-like growth factor I (IGF-I) is a mitogenic polypeptide present in the plasma of man and rat that is thought to mediate the actions of pituitary growth hormone on cartilage to promote skeletal elongation. In the rat, plasma levels of IGF-I show both developmental and hormonal regulation: levels are low at birth, increase with age, and are decreased in growth hormone-deficient adult animals. The present study demonstrates that these changes in plasma IGF-I reflect the abundance of IGF-I RNA in rat liver. A human IGF-I cDNA probe hybridized to multiple RNA species in adult rat liver with sizes 8.6, 4.6, 3.2, 2.1, and 1.0-1.4 kilobases. These RNA species were decreased by >80% in neonatal (2- and 12-day-old) rat liver and by >90% in liver from adult rats made growth hormone-deficient by hypophysectomy. Treatment of hypophysectomized rats with growth hormone increased the abundance of all species of IGF-I RNA. These results suggest that growth hormone regulates the expression of its physiological mediator by altering the synthesis, stability, or both of IGF-I RNA in rat liver.

Original languageEnglish (US)
Pages (from-to)10025-10028
Number of pages4
JournalJournal of Biological Chemistry
Volume261
Issue number22
StatePublished - 1986
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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