TY - JOUR
T1 - Growth Hormone Assessment by Radioreceptor and Radioimmunoassay
T2 - Radioreceptor Assay and Radioimmunoassay Comparisons
AU - Lafranchi, Stephen
AU - Hanna, Cheryl E.
AU - Jelen, Barbara
PY - 1984/1
Y1 - 1984/1
N2 - To investigate possible human growth hormone (HGH) bioinactivity, serum radioreceptor assay (RRA) and radioimmunoassay (RIA) comparisons were made in 48 children undergoing an evaluation for growth retardation. Discrepancies between serum HGH concentrations by RRA and RIA were uncommon; the overall RRA/RIA ratio was 0.75. Significantly reduced HGH RRA/RIA ratios were seen at peak time periods following levodopa and arginine hydrochloride stimulation. Two subjects who demonstrated a discrepancy between serum HGH concentrations by RRA and RIA and somatomedin C levels in the hypopituitary range had malnutrition and chronic disease. There appeared to be an inverse correlation between nutrition, as assessed by a height age—weight age ratio, and RRA/RIA ratios in all subjects. While a reduced RRA/RIA ratio supports the diagnosis of bioinactive HGH, the administration of certain HGH secretagogues, malnutrition, and chronic disease may result in low HGH RRA/RIA ratios.
AB - To investigate possible human growth hormone (HGH) bioinactivity, serum radioreceptor assay (RRA) and radioimmunoassay (RIA) comparisons were made in 48 children undergoing an evaluation for growth retardation. Discrepancies between serum HGH concentrations by RRA and RIA were uncommon; the overall RRA/RIA ratio was 0.75. Significantly reduced HGH RRA/RIA ratios were seen at peak time periods following levodopa and arginine hydrochloride stimulation. Two subjects who demonstrated a discrepancy between serum HGH concentrations by RRA and RIA and somatomedin C levels in the hypopituitary range had malnutrition and chronic disease. There appeared to be an inverse correlation between nutrition, as assessed by a height age—weight age ratio, and RRA/RIA ratios in all subjects. While a reduced RRA/RIA ratio supports the diagnosis of bioinactive HGH, the administration of certain HGH secretagogues, malnutrition, and chronic disease may result in low HGH RRA/RIA ratios.
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U2 - 10.1001/archpedi.1984.02140390015006
DO - 10.1001/archpedi.1984.02140390015006
M3 - Article
C2 - 6537873
AN - SCOPUS:0021326048
SN - 0002-922X
VL - 138
SP - 23
EP - 27
JO - American Journal of Diseases of Children
JF - American Journal of Diseases of Children
IS - 1
ER -