Group I mGlur activation reverses cocaine-induced accumulation of calcium-permeable AMPA receptors in nucleus accumbens synapses via a protein kinase C-dependent mechanism

James E. McCutcheon, Jessica A. Loweth, Kerstin A. Ford, Michela Marinelli, Marina Wolf, Kuei Y. Tseng

Research output: Contribution to journalArticle

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Abstract

Following prolonged withdrawal from extended access cocaine self-administration in adult rats, high conductance Ca 2+-permeable AMPA receptors (CP-AMPARs) accumulate in nucleus accumbens (NAc) synapses and mediate the expression of "incubated" cueinduced cocaine craving. Using patch-clamp recordings from NAc slices prepared after extended access cocaine self-administration and >45 d of withdrawal, we found that group I metabotropic glutamate receptor (mGluR) stimulation using 3,5-dihydroxyphenylglycine (DHPG; 50 μM) rapidly eliminates the postsynaptic CP-AMPAR contribution to NAc synaptic transmission. This is accompanied by facilitation of Ca 2+-impermeable AMPAR (CI-AMPAR)-mediated transmission, suggesting that DHPG may promote an exchange between CP-AMPARs and CI-AMPARs. In saline controls, DHPG also reduced excitatory transmission but this occurred through a CB1 receptor-dependent presynaptic mechanism rather than an effect on postsynapticAMPARs.Blockade of CB1 receptors had no significant effect on the alterations inAMPARtransmission produced byDHPGin the cocaine group. Interestingly, the effect ofDHPGin the cocaine group was mediated by mGluR1 whereas its effect in the saline group was mediated by mGluR5. These results indicate that regulation of synaptic transmission in the NAc is profoundly altered after extended access cocaine self-administration and prolonged withdrawal. Furthermore, they suggest that activation of mGluR1 may represent a potential strategy for reducing cue-induced cocaine craving in abstinent cocaine addicts.

Original languageEnglish (US)
Pages (from-to)14536-14541
Number of pages6
JournalJournal of Neuroscience
Volume31
Issue number41
DOIs
StatePublished - Oct 12 2011
Externally publishedYes

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AMPA Receptors
Nucleus Accumbens
Cocaine
Synapses
Protein Kinase C
Calcium
Self Administration
Cannabinoid Receptor CB1
Synaptic Transmission
Metabotropic Glutamate Receptors
Cues

ASJC Scopus subject areas

  • Neuroscience(all)

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Group I mGlur activation reverses cocaine-induced accumulation of calcium-permeable AMPA receptors in nucleus accumbens synapses via a protein kinase C-dependent mechanism. / McCutcheon, James E.; Loweth, Jessica A.; Ford, Kerstin A.; Marinelli, Michela; Wolf, Marina; Tseng, Kuei Y.

In: Journal of Neuroscience, Vol. 31, No. 41, 12.10.2011, p. 14536-14541.

Research output: Contribution to journalArticle

McCutcheon, James E. ; Loweth, Jessica A. ; Ford, Kerstin A. ; Marinelli, Michela ; Wolf, Marina ; Tseng, Kuei Y. / Group I mGlur activation reverses cocaine-induced accumulation of calcium-permeable AMPA receptors in nucleus accumbens synapses via a protein kinase C-dependent mechanism. In: Journal of Neuroscience. 2011 ; Vol. 31, No. 41. pp. 14536-14541.
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