Gonadotropin-releasing hormone receptor microaggregation. Rate monitored by fluorescence resonance energy transfer

Anda Cornea, Jo Ann Janovick, Guadalupe Maya-Núñez, P. Michael Conn

    Research output: Contribution to journalArticle

    109 Citations (Scopus)

    Abstract

    Gonadotropin-releasing hormone (GnRH) regulates pituitary gonadotropin release and is a therapeutic target for human and animal reproductive diseases. In the present study we have utilized the technique of fluorescence resonance energy transfer to monitor the rate of GnRH receptor-receptor interactions. This technique relies on the observation that the degree of physical intimacy of molecules can be assessed by the tendency of proximal fluorophores to exchange energy. Our data indicate that GnRH agonist, but not antagonist, occupancy of the GnRH receptor promotes physical intimacy (microaggregation) between receptors. The time course indicates that this occurs promptly (

    Original languageEnglish (US)
    Pages (from-to)2153-2158
    Number of pages6
    JournalJournal of Biological Chemistry
    Volume276
    Issue number3
    DOIs
    StatePublished - Jan 19 2001

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    LHRH Receptors
    Fluorescence Resonance Energy Transfer
    Gonadotropin-Releasing Hormone
    Pituitary Gonadotropins
    Animal Diseases
    Fluorophores
    Animals
    Molecules
    Therapeutics

    ASJC Scopus subject areas

    • Biochemistry

    Cite this

    Gonadotropin-releasing hormone receptor microaggregation. Rate monitored by fluorescence resonance energy transfer. / Cornea, Anda; Janovick, Jo Ann; Maya-Núñez, Guadalupe; Conn, P. Michael.

    In: Journal of Biological Chemistry, Vol. 276, No. 3, 19.01.2001, p. 2153-2158.

    Research output: Contribution to journalArticle

    Cornea, Anda ; Janovick, Jo Ann ; Maya-Núñez, Guadalupe ; Conn, P. Michael. / Gonadotropin-releasing hormone receptor microaggregation. Rate monitored by fluorescence resonance energy transfer. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 3. pp. 2153-2158.
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