Glycoproteomics in neurodegenerative diseases

Hyejin Hwang, Jianpeng Zhaang, Kathryn A. Chung, James B. Leverenz, Cyrus P. Zabetian, Elaine R. Peskind, Joseph Jankovic, Zhen Su, Aneeka M. Hancock, Catherine Pan, Thomas J. Montine, Sheng Pan, John Nutt, Roger Albin, Maria Gearing, Richard P. Beyer, Min Shi, Jing Zhang

Research output: Contribution to journalArticle

64 Scopus citations

Abstract

Protein glycosylation regulates protein function and cellular distribution. Additionally, aberrant protein glycosylations have been recognized to play major roles in human disorders, including neurodegenerative diseases. Glycoproteomics, a branch of proteomics that catalogs and quantifies glycoproteins, provides a powerful means to systematically profile the glycopeptides or glycoproteins of a complex mixture that are highly enriched in body fluids, and therefore, carry great potential to be diagnostic and/or prognostic markers. Application of this mass spectrometry-based technology to the study of neurodegenerative disorders (e.g., Alzheimer's disease and Parkinson's disease) is relatively new, and is expected to provide insight into the biochemical pathogenesis of neurodegeneration, as well as biomarker discovery. In this review, we have summarized the current understanding of glycoproteins in biology and neurodegenerative disease, and have discussed existing proteomic technologies that are utilized to characterize glycoproteins. Some of the ongoing studies, where glycoproteins isolated from cerebrospinal fluid and human brain are being characterized in Parkinson's disease at different stages versus controls, are presented, along with future applications of targeted validation of brain specific glycoproteins in body fluids.

Original languageEnglish (US)
Pages (from-to)79-125
Number of pages47
JournalMass Spectrometry Reviews
Volume29
Issue number1
DOIs
StatePublished - Jan 2010

Keywords

  • Alzheimer's diseases
  • Biomarkers
  • Cerebrospinal fluids
  • Glycoproteomics
  • Mass spectrometry
  • Parkinson's disease

ASJC Scopus subject areas

  • Analytical Chemistry
  • Condensed Matter Physics
  • Biochemistry, Genetics and Molecular Biology(all)
  • Spectroscopy

Fingerprint Dive into the research topics of 'Glycoproteomics in neurodegenerative diseases'. Together they form a unique fingerprint.

  • Cite this

    Hwang, H., Zhaang, J., Chung, K. A., Leverenz, J. B., Zabetian, C. P., Peskind, E. R., Jankovic, J., Su, Z., Hancock, A. M., Pan, C., Montine, T. J., Pan, S., Nutt, J., Albin, R., Gearing, M., Beyer, R. P., Shi, M., & Zhang, J. (2010). Glycoproteomics in neurodegenerative diseases. Mass Spectrometry Reviews, 29(1), 79-125. https://doi.org/10.1002/mas.20221