Glucocorticoid receptors in Epstein-Barr virus-transformed human lymphocytes

M. Tomita, G. P. Chrousos, D. D. Brandon, S. Ben-Or, C. M. Foster, L. De Vougn, S. Taylor, D. L. Loriaux, M. B. Lipsett

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Glucocorticoid receptors were studied in cultured human lymphocytes from normal donors after transformation with the Epstein-Barr Virus (EBV) and compared to those of circulating human mononuclear leukocytes. Both whole cell and cytosol fractions were examined for [3H]-dexamethasone binding. The concentration and absolute number of glucocorticoid binding sites were increased five-fold in the transformed cells when compared to the non-transformed human mononuclear leukocytes. However, the affinity (Kd) of the glucocorticoid receptor for dexamethasone was the same in both types of cells. The denatured glucocorticoid receptor, covalently labelled with [3H]-dexamethasone-21-mesylate, was identified by SDS polyacrylamide gel electrophoresis as a protein moiety with M(r)~92,000, similar to that obtained from human non-transformed mononuclear leukocytes. The pattern of the activation of the hormone-receptor complexes, analyzed by phosphocellulose chromatography, was similar in both types of cells, and also the time-courses of loss of specific binding during thermal activation were similar. These results suggest that viral transformation is associated with increases in the concentration and the absolute number of glucocorticoid receptors whereas other qualitative receptor characteristics remain similar. Thus, transformation of cells with EB virus can provide a constant source of glucocorticoid receptors for study.

Original languageEnglish (US)
Pages (from-to)674-678
Number of pages5
JournalHormone and Metabolic Research
Volume17
Issue number12
DOIs
StatePublished - Jan 1 1985

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

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    Tomita, M., Chrousos, G. P., Brandon, D. D., Ben-Or, S., Foster, C. M., De Vougn, L., Taylor, S., Loriaux, D. L., & Lipsett, M. B. (1985). Glucocorticoid receptors in Epstein-Barr virus-transformed human lymphocytes. Hormone and Metabolic Research, 17(12), 674-678. https://doi.org/10.1055/s-2007-1013641