@article{f45aad1365464b35871fe6ca8da980c1,
title = "Global profiling of stimulus-induced polyadenylation in cells using a poly(A) trap",
abstract = "Polyadenylation of mRNA leads to increased protein expression in response to diverse stimuli, but it is difficult to identify mRNAs that become polyadenylated in living cells. Here we describe a click chemistry-compatible nucleoside analog that is selectively incorporated into poly(A) tails of transcripts in cells. Next-generation sequencing of labeled mRNAs enables a transcriptome-wide profile of polyadenylation and provides insights into the mRNA sequence elements that are correlated with polyadenylation.",
author = "Dusica Curanovic and Michael Cohen and Irtisha Singh and Slagle, {Christopher E.} and Leslie, {Christina S.} and Jaffrey, {Samie R.}",
note = "Funding Information: We thank members of the Jaffrey lab for helpful comments and suggestions. We thank J. Richter (University of Massachusetts Medical School) for the antibody to CPEB. We gratefully acknowledge C. Bracken and N. Svensen for assistance with NMR spectroscopy. This work was supported by US National Institutes of Health–National Institute on Drug Abuse grant T32DA007274 (D.C. and M.C.) and US National Institute of Neurological Disorders and Stroke grant R01NS56306 (S.R.J.).",
year = "2013",
month = nov,
doi = "10.1038/nchembio.1334",
language = "English (US)",
volume = "9",
pages = "671--673",
journal = "Nature Chemical Biology",
issn = "1552-4450",
publisher = "Nature Publishing Group",
number = "11",
}