Global profiling of stimulus-induced polyadenylation in cells using a poly(A) trap

Dusica Curanovic, Michael Cohen, Irtisha Singh, Christopher E. Slagle, Christina S. Leslie, Samie R. Jaffrey

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Polyadenylation of mRNA leads to increased protein expression in response to diverse stimuli, but it is difficult to identify mRNAs that become polyadenylated in living cells. Here we describe a click chemistry-compatible nucleoside analog that is selectively incorporated into poly(A) tails of transcripts in cells. Next-generation sequencing of labeled mRNAs enables a transcriptome-wide profile of polyadenylation and provides insights into the mRNA sequence elements that are correlated with polyadenylation.

Original languageEnglish (US)
Pages (from-to)671-673
Number of pages3
JournalNature Chemical Biology
Volume9
Issue number11
DOIs
StatePublished - Nov 2013

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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