Genomic loci and candidate genes underlying inflammatory nociception

Harsha K. Nair, Heather Hain, Raymond M. Quock, Vivek M. Philip, Elissa J. Chesler, John Belknap, William R. Lariviere

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Heritable genetic factors contribute significantly to inflammatory nociception. To determine candidate genes underlying inflammatory nociception, the current study used a mouse model of abdominal inflammatory pain. BXD recombinant inbred (RI) mouse strains were administered the intraperitoneal acetic acid test, and genome-wide quantitative trait locus (QTL) mapping was performed on the mean number of abdominal contraction and extension movements in 3 distinct groups of BXD RI mouse strains in 2 separate experiments. Combined mapping results detected 2 QTLs on chromosomes (Chr) 3 and 10 across experiments and groups of mice; an additional sex-specific QTL was detected on Chr 16. The results replicate previous findings of a significant QTL, Nociq2, on distal Chr 10 for formalin-induced inflammatory nociception and will aid in identification of the underlying candidate genes. Comparisons of sensitivity to intraperitoneal acetic acid in BXD RI mouse strains with microarray mRNA transcript expression profiles in specific brain areas detected covarying expression of candidate genes that are also found in the detected QTL confidence intervals. The results indicate that common and distinct genetic mechanisms underlie heritable sensitivity to diverse inflammatory insults, and provide a discrete set of high-priority candidate genes to investigate further in rodents and human association studies. Novel genomic regions linked to inflammatory nociception were detected, a previously reported locus was confirmed, and high-priority candidate genes for inflammatory nociception and pain were identified.

Original languageEnglish (US)
Pages (from-to)599-606
Number of pages8
JournalPain
Volume152
Issue number3
DOIs
StatePublished - Mar 2011

Fingerprint

Nociception
Quantitative Trait Loci
Inbred Strains Mice
Chromosomes, Human, Pair 10
Genes
Acetic Acid
Chromosomes, Human, Pair 16
Chromosomes, Human, Pair 3
Genetic Association Studies
Abdominal Pain
Formaldehyde
Rodentia
Genome
Confidence Intervals
Gene Expression
Pain
Messenger RNA
Brain

Keywords

  • Acetic acid
  • Inflammation
  • Microarray
  • Nociception
  • Quantitative trait locus mapping
  • Transcript abundance

ASJC Scopus subject areas

  • Clinical Neurology
  • Anesthesiology and Pain Medicine
  • Neurology
  • Pharmacology

Cite this

Nair, H. K., Hain, H., Quock, R. M., Philip, V. M., Chesler, E. J., Belknap, J., & Lariviere, W. R. (2011). Genomic loci and candidate genes underlying inflammatory nociception. Pain, 152(3), 599-606. https://doi.org/10.1016/j.pain.2010.11.029

Genomic loci and candidate genes underlying inflammatory nociception. / Nair, Harsha K.; Hain, Heather; Quock, Raymond M.; Philip, Vivek M.; Chesler, Elissa J.; Belknap, John; Lariviere, William R.

In: Pain, Vol. 152, No. 3, 03.2011, p. 599-606.

Research output: Contribution to journalArticle

Nair, HK, Hain, H, Quock, RM, Philip, VM, Chesler, EJ, Belknap, J & Lariviere, WR 2011, 'Genomic loci and candidate genes underlying inflammatory nociception', Pain, vol. 152, no. 3, pp. 599-606. https://doi.org/10.1016/j.pain.2010.11.029
Nair HK, Hain H, Quock RM, Philip VM, Chesler EJ, Belknap J et al. Genomic loci and candidate genes underlying inflammatory nociception. Pain. 2011 Mar;152(3):599-606. https://doi.org/10.1016/j.pain.2010.11.029
Nair, Harsha K. ; Hain, Heather ; Quock, Raymond M. ; Philip, Vivek M. ; Chesler, Elissa J. ; Belknap, John ; Lariviere, William R. / Genomic loci and candidate genes underlying inflammatory nociception. In: Pain. 2011 ; Vol. 152, No. 3. pp. 599-606.
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