Genomic imprinting in primate embryos and embryonic stem cells

    Research output: Contribution to journalArticle

    19 Citations (Scopus)

    Abstract

    Embryonic stem (ES) cells hold promise for cell and tissue replacement approaches to treating human diseases. However, long-term in vitro culture and manipulations of ES cells may adversely affect their epigenetic integrity including imprinting. Disruption or inappropriate expression of imprinted genes is associated with several clinically significant syndromes and tumorigenesis in humans. We demonstrated aberrant biallelic expression of IGF2 and H19 in several rhesus monkey ES cell lines while SNRPN and NDN were normally imprinted and expressed from the paternal allele. In contrast, expanded blastocyst-stage embryos, from which these ES cells were derived, exhibited normal paternal expression of IGF2 and maternal expression of H19. To test the possibility that aberrant methylation at an imprinting centre (IC) upstream of H19 accounts for the relaxed imprinting of IGF2 and H19, we performed comprehensive methylation analysis by investigating methylation profiles of CpG sites within the IGF2/H19 IC. Our results demonstrate abnormal hypermethylation within the IGF2/H19 IC in all analysed ES cell lines consistent with biallelic expression of these genes. Cellular overproliferation and tumour formation resulting from tissue or cell transplantation are potential problems that must be addressed before clinical trials of ES cell-based therapy are initiated.

    Original languageEnglish (US)
    Pages (from-to)817-821
    Number of pages5
    JournalReproduction, Fertility and Development
    Volume18
    Issue number8
    DOIs
    StatePublished - 2006

    Fingerprint

    Genomic Imprinting
    genomic imprinting
    embryonic stem cells
    Embryonic Stem Cells
    Primates
    embryo (animal)
    Embryonic Structures
    methylation
    Methylation
    snRNP Core Proteins
    tissue transplantation
    cell lines
    Gene Expression
    Tissue Transplantation
    Cell Line
    gene expression
    cell transplantation
    Cell Transplantation
    Blastocyst
    Cell- and Tissue-Based Therapy

    Keywords

    • Methylation
    • Rhesus monkey

    ASJC Scopus subject areas

    • Animal Science and Zoology
    • Reproductive Medicine
    • Developmental Biology
    • Endocrinology

    Cite this

    Genomic imprinting in primate embryos and embryonic stem cells. / Mitalipov, Shoukhrat.

    In: Reproduction, Fertility and Development, Vol. 18, No. 8, 2006, p. 817-821.

    Research output: Contribution to journalArticle

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